Chapter title |
Fractionation Techniques to Examine Effector Translocation.
|
---|---|
Chapter number | 9 |
Book title |
Type 3 Secretion Systems
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6649-3_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6647-9, 978-1-4939-6649-3
|
Authors |
Rachel M. Olson, Deborah M. Anderson |
Editors |
Matthew L. Nilles, Danielle L. Jessen Condry |
Abstract |
Many Gram-negative bacterial pathogens use type III secretion systems to export proteins that act directly on the host and aid in the infectious process. Extracellular bacteria primarily rely upon the type III secretion system to insert or inject effector proteins into the cytosol of their host cell in order to perturb intracellular signaling events and aid in pathogenesis. Intracellular bacteria can also depend on the T3SS translocation of effector proteins from vacuolar compartments into the vacuolar membrane or host cell cytosol where they can modulate intracellular trafficking and/or signaling pathways necessary for their growth and survival. Biochemical fractionation of infected cells in vitro enables detection of these events, making it possible to identify relevant protein-protein interactions, characterize phenotypes of mutant strains and understand how these effector proteins impact host cells. In this chapter we provide methods for the analysis of translocated effector proteins using biochemical and mechanical fractionation procedures. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 3 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 2 | 67% |
Student > Doctoral Student | 1 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 1 | 33% |
Agricultural and Biological Sciences | 1 | 33% |
Immunology and Microbiology | 1 | 33% |