| Chapter title |
T2 Magnetic Resonance for Fungal Diagnosis.
|
|---|---|
| Chapter number | 18 |
| Book title |
Human Fungal Pathogen Identification
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6515-1_18 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6513-7, 978-1-4939-6515-1
|
| Authors |
Fainareti N. Zervou, Ioannis M. Zacharioudakis, Jaclynn Kurpewski, Eleftherios Mylonakis M.D., Ph.D., F.I.D.S.A., Eleftherios Mylonakis |
| Editors |
Thomas Lion |
| Abstract |
Rapid diagnostic methods for fungal infections are long awaited and are expected to improve outcomes through early initiation of targeted antifungal therapy. T2Candida panel is a novel qualitative diagnostic platform that was recently approved by the US Food and Drug Administration (FDA) for diagnosis of candidemia with a mean time to species identification of less than 5 h. T2Candida panel is performed on the fully automated T2Dx instrument in whole blood K2EDTA specimens and is able to detect 5 Candida spp., namely Candida albicans, Candida tropicalis, Candida parapsilosis, Candida krusei, and Candida glabrata. By combining magnetic resonance with molecular diagnostics, T2Candida panel amplifies DNA and detects the amplified product by amplicon-induced agglomeration of supermagnetic particles and T2 Magnetic Resonance (T2MR) measurement. Here we describe the materials and methods needed to diagnose candidemia with the T2Candida panel. |
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Geographical breakdown
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|---|---|---|
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Demographic breakdown
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|---|---|---|
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| Student > Bachelor | 4 | 12% |
| Student > Ph. D. Student | 4 | 12% |
| Researcher | 2 | 6% |
| Student > Postgraduate | 2 | 6% |
| Other | 3 | 9% |
| Unknown | 13 | 38% |
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|---|---|---|
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| Agricultural and Biological Sciences | 4 | 12% |
| Pharmacology, Toxicology and Pharmaceutical Science | 3 | 9% |
| Immunology and Microbiology | 2 | 6% |
| Other | 4 | 12% |
| Unknown | 11 | 32% |