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Mendeley readers
Attention Score in Context
| Title |
A New Fluorescence-Based Method Identifies Protein Phosphatases Regulating Lipid Droplet Metabolism
|
|---|---|
| Published in |
PLOS ONE, October 2010
|
| DOI | 10.1371/journal.pone.0013692 |
| Pubmed ID | |
| Authors |
Bruno L. Bozaquel-Morais, Juliana B. Madeira, Clarissa M. Maya-Monteiro, Claudio A. Masuda, Mónica Montero-Lomeli |
| Abstract |
In virtually every cell, neutral lipids are stored in cytoplasmic structures called lipid droplets (LDs) and also referred to as lipid bodies or lipid particles. We developed a rapid high-throughput assay based on the recovery of quenched BODIPY-fluorescence that allows to quantify lipid droplets. The method was validated by monitoring lipid droplet turnover during growth of a yeast culture and by screening a group of strains deleted in genes known to be involved in lipid metabolism. In both tests, the fluorimetric assay showed high sensitivity and good agreement with previously reported data using microscopy. We used this method for high-throughput identification of protein phosphatases involved in lipid droplet metabolism. From 65 yeast knockout strains encoding protein phosphatases and its regulatory subunits, 13 strains revealed to have abnormal levels of lipid droplets, 10 of them having high lipid droplet content. Strains deleted for type I protein phosphatases and related regulators (ppz2, gac1, bni4), type 2A phosphatase and its related regulator (pph21 and sap185), type 2C protein phosphatases (ptc1, ptc4, ptc7) and dual phosphatases (pps1, msg5) were catalogued as high-lipid droplet content strains. Only reg1, a targeting subunit of the type 1 phosphatase Glc7p, and members of the nutrient-sensitive TOR pathway (sit4 and the regulatory subunit sap190) were catalogued as low-lipid droplet content strains, which were studied further. We show that Snf1, the homologue of the mammalian AMP-activated kinase, is constitutively phosphorylated (hyperactive) in sit4 and sap190 strains leading to a reduction of acetyl-CoA carboxylase activity. In conclusion, our fast and highly sensitive method permitted us to catalogue protein phosphatases involved in the regulation of LD metabolism and present evidence indicating that the TOR pathway and the SNF1/AMPK pathway are connected through the Sit4p-Sap190p pair in the control of lipid droplet biogenesis. |
Mendeley readers
The data shown below were compiled from readership statistics for 146 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Austria | 2 | 1% |
| Spain | 2 | 1% |
| Czechia | 1 | <1% |
| Brazil | 1 | <1% |
| Egypt | 1 | <1% |
| United States | 1 | <1% |
| Unknown | 138 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 32 | 22% |
| Student > Ph. D. Student | 29 | 20% |
| Student > Master | 18 | 12% |
| Student > Bachelor | 11 | 8% |
| Professor > Associate Professor | 10 | 7% |
| Other | 27 | 18% |
| Unknown | 19 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 66 | 45% |
| Biochemistry, Genetics and Molecular Biology | 39 | 27% |
| Engineering | 5 | 3% |
| Medicine and Dentistry | 3 | 2% |
| Business, Management and Accounting | 2 | 1% |
| Other | 8 | 5% |
| Unknown | 23 | 16% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 April 2013.
All research outputs
#20,191,579
of 22,708,120 outputs
Outputs from PLOS ONE
#173,043
of 193,897 outputs
Outputs of similar age
#93,957
of 99,312 outputs
Outputs of similar age from PLOS ONE
#915
of 941 outputs
Altmetric has tracked 22,708,120 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 193,897 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.0. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 99,312 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 941 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.