Chapter title |
Human Induced Pluripotent Stem Cell-Derived Microglia (hiPSC-Microglia).
|
---|---|
Chapter number | 429 |
Book title |
Induced Pluripotent Stem (iPS) Cells
|
Published in |
Methods in molecular biology, November 2021
|
DOI | 10.1007/7651_2021_429 |
Pubmed ID | |
Book ISBNs |
978-1-07-162118-9, 978-1-07-162119-6
|
Authors |
McQuade, Amanda, Blurton-Jones, Mathew |
Abstract |
Microglia are crucial mediators of brain development and homeostasis. In recent years, it has been additionally suggested that modulation of microglial function may prove to be a useful therapeutic technique in many neurological contexts. However, before we can develop therapeutics, we need to better understand homeostatic microglial processes at the cellular and molecular level. For this reason, it has become crucial to develop better models to study human microglia which are known to be quite distinct from murine models. Here we provide a detailed method to differentiate homeostatic microglia from human pluripotent cells. Additionally, due to the innate sensitivity of these immune cells, we have provided detailed notes for best practices of handling cultured microglia. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 17 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 3 | 18% |
Other | 2 | 12% |
Student > Doctoral Student | 2 | 12% |
Student > Bachelor | 2 | 12% |
Student > Master | 1 | 6% |
Other | 2 | 12% |
Unknown | 5 | 29% |
Readers by discipline | Count | As % |
---|---|---|
Neuroscience | 4 | 24% |
Biochemistry, Genetics and Molecular Biology | 1 | 6% |
Immunology and Microbiology | 1 | 6% |
Agricultural and Biological Sciences | 1 | 6% |
Medicine and Dentistry | 1 | 6% |
Other | 1 | 6% |
Unknown | 8 | 47% |