Chapter title |
NIRVANA for Simultaneous Detection and Mutation Surveillance of SARS-CoV-2 and Co-infections of Multiple Respiratory Viruses.
|
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Chapter number | 6 |
Book title |
Multiplex Biomarker Techniques
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Published in |
Methods in molecular biology, January 2022
|
DOI | 10.1007/978-1-0716-2395-4_6 |
Pubmed ID | |
Book ISBNs |
978-1-07-162394-7, 978-1-07-162395-4
|
Authors |
Bi, Chongwei, Ramos-Mandujano, Gerardo, Li, Mo |
Abstract |
Detection and mutation surveillance of SARS-CoV-2 are crucial for combating the COVID-19 pandemic. Here we describe a lab-based method for multiplex isothermal amplification-based sequencing and real-time analysis of multiple viral genomes. It can simultaneously detect SARS-CoV-2, influenza A, human adenovirus, and human coronavirus and monitor mutations for up to 96 samples in real time. The method proved to be rapid and sensitive (limit of detection: 29 viral RNA copies/μL of extracted nucleic acid) in detecting SARS-CoV-2 in clinical samples. We expect it to offer a promising solution for rapid field-deployable detection and mutational surveillance of pandemic viruses. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Saudi Arabia | 2 | 50% |
Unknown | 2 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 3 | 75% |
Scientists | 1 | 25% |