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Nucleic Acid Aptamers

Overview of attention for book
Cover of 'Nucleic Acid Aptamers'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Automated ssDNA SELEX Using Histidine-Tagged Target Proteins
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    Chapter 2 Discovery of Aptamers Against Cell Surface Markers Using Ligand-Guided Selection
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    Chapter 3 Implementation of Emulsion PCR for Amplification of Click-Modified DNA During SELEX
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    Chapter 4 Preparation of Chemically Modified DNA Library for SELEX via Incorporation of CLB-dUTP in Primer Extension Reaction
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    Chapter 5 RNA Capture-SELEX on Streptavidin Magnetic Beads
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    Chapter 6 The Bioinformatics of Aptamers: HT-SELEX Analysis with AptaSUITE
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    Chapter 7 Improvement of Aptamers by High-Throughput Sequencing of Doped-SELEX
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    Chapter 8 Analysis of Aptamer-Small Molecule Binding Interactions Using Isothermal Titration Calorimetry
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    Chapter 9 Determination of Aptamer Structure Using Circular Dichroism Spectroscopy
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    Chapter 10 Immobilization-Free Determination of Dissociation Constants Independent of Ligand Size Using MicroScale Thermophoresis
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    Chapter 11 Label-Free Determination of the Kinetic Parameters of Protein-Aptamer Interaction by Surface Plasmon Resonance
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    Chapter 12 Probing of Fluorogenic RNA Aptamers via Supramolecular Förster Resonance Energy Transfer with a Universal Fluorescent Nucleobase Analog
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    Chapter 13 Slow-Off-Rate-Modified Aptamer Labeling for Fluorescence Microscopy and DNA-PAINT
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    Chapter 14 Aptamer–Field-Effect Transistors for Small-Molecule Sensing in Complex Environments
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    Chapter 15 Aptasensor for Impedimetric Detection of Lysozyme
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    Chapter 16 Biophysical Analysis of Small Molecule Binding to Viral RNA Structures
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    Chapter 17 Designing and Expressing Circular RNA Aptamers to Regulate Mammalian Cell Biology
  19. Altmetric Badge
    Chapter 18 Enzyme-Linked Oligonucleotide Assay (ELONA)
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    Chapter 19 High-Throughput Development and Optimization of RNA-Based Fluorogenic Biosensors of Small Molecules Using Droplet-Based Microfluidics
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    Chapter 20 Robotic APTamer-Enabled Electrochemical Reader (RAPTER) System for Automated Aptamer-Mediated Electrochemical Analysis
Attention for Chapter 13: Slow-Off-Rate-Modified Aptamer Labeling for Fluorescence Microscopy and DNA-PAINT
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Chapter title
Slow-Off-Rate-Modified Aptamer Labeling for Fluorescence Microscopy and DNA-PAINT
Chapter number 13
Book title
Nucleic Acid Aptamers
Published in
Methods in molecular biology, January 2023
DOI 10.1007/978-1-0716-2695-5_13
Pubmed ID
Book ISBNs
978-1-07-162694-8, 978-1-07-162695-5
Authors

Strauss, Sebastian, Jungmann, Ralf

Abstract

Super-resolution microscopy methods enable the visualization of biological processes on the level of a few nanometers. However, the application of these techniques in biological systems is limited by the availability of small affinity reagents. Slow off-rate-modified aptamers as nucleic acid analogues to antibodies have been successfully applied to improve the resolution and quantification of DNA-PAINT. In this chapter, we describe a protocol for using SOMAmers as labeling reagents for super-resolution microscopy.

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Mendeley readers

The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 3 100%

Demographic breakdown

Readers by professional status Count As %
Student > Doctoral Student 2 67%
Student > Ph. D. Student 1 33%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 67%
Nursing and Health Professions 1 33%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 September 2022.
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#19,250,592
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Outputs from Methods in molecular biology
#7,934
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Outputs of similar age from Methods in molecular biology
#489
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