Chapter title |
Protocol of Detection of West Nile Virus in Clinical Samples.
|
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Chapter number | 12 |
Book title |
West Nile Virus
|
Published in |
Methods in molecular biology, January 2023
|
DOI | 10.1007/978-1-0716-2760-0_12 |
Pubmed ID | |
Book ISBNs |
978-1-07-162759-4, 978-1-07-162760-0
|
Authors |
Nwanosike, Hephzibah, Green, Freedom M, Murray, Kristy O, Weatherhead, Jill E, Ronca, Shannon E, Green, Freedom M., Murray, Kristy O., Weatherhead, Jill E., Ronca, Shannon E. |
Abstract |
West Nile virus (WNV) is one of the leading causes of arboviral encephalitis in the United States but is often underdiagnosed. Despite the wide breadth of WNV-induced clinical disease syndromes, many of the symptoms associated with WNV are nonspecific at the time of presentation; thus, choosing the right diagnostic tool is essential to not only understand the true burden of disease but also provide pathogen-directed interventions for WNV-infected patients. In this chapter, we briefly discuss the three most common types of diagnostic methods for WNV in human clinical samples: nucleic acid detection, enzyme-linked immunoassay (ELISA), and plaque reduction neutralization test (PRNT) and present the method for PRNT. |
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