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Utilizing functional cell‐free extracts to dissect ribonucleoprotein complex biology at single‐molecule resolution

Overview of attention for article published in Wiley Interdisciplinary Reviews: RNA, April 2023
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Title
Utilizing functional cell‐free extracts to dissect ribonucleoprotein complex biology at single‐molecule resolution
Published in
Wiley Interdisciplinary Reviews: RNA, April 2023
DOI 10.1002/wrna.1787
Pubmed ID
Authors

Elizabeth Duran, Andreas Schmidt, Robb Welty, Ameya P. Jalihal, Sethuramasundaram Pitchiaya, Nils G. Walter

Abstract

Cellular machineries that drive and regulate gene expression often rely on the coordinated assembly and interaction of a multitude of proteins and RNA together called ribonucleoprotein complexes (RNPs). As such, it is challenging to fully reconstitute these cellular machines recombinantly and gain mechanistic understanding of how they operate and are regulated within the complex environment that is the cell. One strategy for overcoming this challenge is to perform single molecule fluorescence microscopy studies within crude or recombinantly supplemented cell extracts. This strategy enables elucidation of the interaction and kinetic behavior of specific fluorescently labeled biomolecules within RNPs under conditions that approximate native cellular environments. In this review, we describe single molecule fluorescence microcopy approaches that dissect RNP-driven processes within cellular extracts, highlighting general strategies used in these methods. We further survey biological advances in the areas of pre-mRNA splicing and transcription regulation that have been facilitated through this approach. Finally, we conclude with a summary of practical considerations for the implementation of the featured approaches to facilitate their broader future implementation in dissecting the mechanisms of RNP-driven cellular processes. This article is categorized under: RNA Structure and Dynamics > RNA Structure, Dynamics and Chemistry RNA Interactions with Proteins and Other Molecules > RNA-Protein Complexes RNA Structure and Dynamics > Influence of RNA Structure in Biological Systems.

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Mendeley readers

The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 2 100%

Demographic breakdown

Readers by professional status Count As %
Professor 1 50%
Researcher 1 50%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 100%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 April 2023.
All research outputs
#15,337,507
of 23,578,918 outputs
Outputs from Wiley Interdisciplinary Reviews: RNA
#401
of 652 outputs
Outputs of similar age
#131,200
of 259,409 outputs
Outputs of similar age from Wiley Interdisciplinary Reviews: RNA
#4
of 5 outputs
Altmetric has tracked 23,578,918 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 652 research outputs from this source. They receive a mean Attention Score of 4.9. This one is in the 32nd percentile – i.e., 32% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 259,409 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 46th percentile – i.e., 46% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 5 others from the same source and published within six weeks on either side of this one.