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Site-Specific Phosphorylation of the DNA Damage Response Mediator Rad9 by Cyclin-Dependent Kinases Regulates Activation of Checkpoint Kinase 1

Overview of attention for article published in PLoS Genetics, April 2013
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Title
Site-Specific Phosphorylation of the DNA Damage Response Mediator Rad9 by Cyclin-Dependent Kinases Regulates Activation of Checkpoint Kinase 1
Published in
PLoS Genetics, April 2013
DOI 10.1371/journal.pgen.1003310
Pubmed ID
Authors

Carla Manuela Abreu, Ramesh Kumar, Danielle Hamilton, Andrew William Dawdy, Kevin Creavin, Sarah Eivers, Karen Finn, Jeremy Lynn Balsbaugh, Rosemary O'Connor, Patrick A. Kiely, Jeffrey Shabanowitz, Donald F. Hunt, Muriel Grenon, Noel Francis Lowndes

Abstract

The mediators of the DNA damage response (DDR) are highly phosphorylated by kinases that control cell proliferation, but little is known about the role of this regulation. Here we show that cell cycle phosphorylation of the prototypical DDR mediator Saccharomyces cerevisiae Rad9 depends on cyclin-dependent kinase (CDK) complexes. We find that a specific G2/M form of Cdc28 can phosphorylate in vitro the N-terminal region of Rad9 on nine consensus CDK phosphorylation sites. We show that the integrity of CDK consensus sites and the activity of Cdc28 are required for both the activation of the Chk1 checkpoint kinase and its interaction with Rad9. We have identified T125 and T143 as important residues in Rad9 for this Rad9/Chk1 interaction. Phosphorylation of T143 is the most important feature promoting Rad9/Chk1 interaction, while the much more abundant phosphorylation of the neighbouring T125 residue impedes the Rad9/Chk1 interaction. We suggest a novel model for Chk1 activation where Cdc28 regulates the constitutive interaction of Rad9 and Chk1. The Rad9/Chk1 complex is then recruited at sites of DNA damage where activation of Chk1 requires additional DDR-specific protein kinases.

Mendeley readers

The data shown below were compiled from readership statistics for 58 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Japan 1 2%
United States 1 2%
Ireland 1 2%
Unknown 55 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 16 28%
Researcher 13 22%
Student > Master 8 14%
Student > Bachelor 5 9%
Professor > Associate Professor 4 7%
Other 6 10%
Unknown 6 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 25 43%
Biochemistry, Genetics and Molecular Biology 18 31%
Arts and Humanities 2 3%
Chemistry 2 3%
Environmental Science 1 2%
Other 2 3%
Unknown 8 14%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 April 2013.
All research outputs
#10,722,127
of 12,091,568 outputs
Outputs from PLoS Genetics
#5,739
of 6,176 outputs
Outputs of similar age
#111,718
of 133,965 outputs
Outputs of similar age from PLoS Genetics
#151
of 163 outputs
Altmetric has tracked 12,091,568 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 6,176 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 13.5. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 133,965 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 163 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.