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Mendeley readers
Attention Score in Context
| Title |
Efficient Feeder-Free Episomal Reprogramming with Small Molecules
|
|---|---|
| Published in |
PLOS ONE, March 2011
|
| DOI | 10.1371/journal.pone.0017557 |
| Pubmed ID | |
| Authors |
Junying Yu, Kevin Fongching Chau, Maxim A. Vodyanik, Jinlan Jiang, Yong Jiang |
| Abstract |
Genetic reprogramming of human somatic cells to induced pluripotent stem cells (iPSCs) could offer replenishable cell sources for transplantation therapies. To fulfill their promises, human iPSCs will ideally be free of exogenous DNA (footprint-free), and be derived and cultured in chemically defined media free of feeder cells. Currently, methods are available to enable efficient derivation of footprint-free human iPSCs. However, each of these methods has its limitations. We have previously derived footprint-free human iPSCs by employing episomal vectors for transgene delivery, but the process was inefficient and required feeder cells. Here, we have greatly improved the episomal reprogramming efficiency using a cocktail containing MEK inhibitor PD0325901, GSK3β inhibitor CHIR99021, TGF-β/Activin/Nodal receptor inhibitor A-83-01, ROCK inhibitor HA-100 and human leukemia inhibitory factor. Moreover, we have successfully established a feeder-free reprogramming condition using chemically defined medium with bFGF and N2B27 supplements and chemically defined human ESC medium mTeSR1 for the derivation of footprint-free human iPSCs. These improvements enabled the routine derivation of footprint-free human iPSCs from skin fibroblasts, adipose tissue-derived cells and cord blood cells. This technology will likely be valuable for the production of clinical-grade human iPSCs. |
Mendeley readers
The data shown below were compiled from readership statistics for 309 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 6 | 2% |
| Brazil | 5 | 2% |
| Germany | 2 | <1% |
| Switzerland | 1 | <1% |
| United Kingdom | 1 | <1% |
| France | 1 | <1% |
| China | 1 | <1% |
| Mexico | 1 | <1% |
| Unknown | 291 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 78 | 25% |
| Researcher | 65 | 21% |
| Student > Bachelor | 31 | 10% |
| Student > Master | 29 | 9% |
| Professor | 19 | 6% |
| Other | 56 | 18% |
| Unknown | 31 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 147 | 48% |
| Biochemistry, Genetics and Molecular Biology | 49 | 16% |
| Medicine and Dentistry | 36 | 12% |
| Neuroscience | 14 | 5% |
| Engineering | 9 | 3% |
| Other | 16 | 5% |
| Unknown | 38 | 12% |
Attention Score in Context
This research output has an Altmetric Attention Score of 15. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 February 2024.
All research outputs
#2,235,643
of 24,292,134 outputs
Outputs from PLOS ONE
#27,714
of 209,330 outputs
Outputs of similar age
#9,488
of 112,712 outputs
Outputs of similar age from PLOS ONE
#239
of 1,400 outputs
Altmetric has tracked 24,292,134 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 90th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 209,330 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.6. This one has done well, scoring higher than 86% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 112,712 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 91% of its contemporaries.
We're also able to compare this research output to 1,400 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 82% of its contemporaries.