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Mendeley readers
Attention Score in Context
| Title |
High Log-Scale Expansion of Functional Human Natural Killer Cells from Umbilical Cord Blood CD34-Positive Cells for Adoptive Cancer Immunotherapy
|
|---|---|
| Published in |
PLOS ONE, February 2010
|
| DOI | 10.1371/journal.pone.0009221 |
| Pubmed ID | |
| Authors |
Jan Spanholtz, Marleen Tordoir, Diana Eissens, Frank Preijers, Arnold van der Meer, Irma Joosten, Nicolaas Schaap, Theo M. de Witte, Harry Dolstra |
| Abstract |
Immunotherapy based on natural killer (NK) cell infusions is a potential adjuvant treatment for many cancers. Such therapeutic application in humans requires large numbers of functional NK cells that have been selected and expanded using clinical grade protocols. We established an extremely efficient cytokine-based culture system for ex vivo expansion of NK cells from hematopoietic stem and progenitor cells from umbilical cord blood (UCB). Systematic refinement of this two-step system using a novel clinical grade medium resulted in a therapeutically applicable cell culture protocol. CD56(+)CD3(-) NK cell products could be routinely generated from freshly selected CD34(+) UCB cells with a mean expansion of >15,000 fold and a nearly 100% purity. Moreover, our protocol has the capacity to produce more than 3-log NK cell expansion from frozen CD34(+) UCB cells. These ex vivo-generated cell products contain NK cell subsets differentially expressing NKG2A and killer immunoglobulin-like receptors. Furthermore, UCB-derived CD56(+) NK cells generated by our protocol uniformly express high levels of activating NKG2D and natural cytotoxicity receptors. Functional analysis showed that these ex vivo-generated NK cells efficiently target myeloid leukemia and melanoma tumor cell lines, and mediate cytolysis of primary leukemia cells at low NK-target ratios. Our culture system exemplifies a major breakthrough in producing pure NK cell products from limited numbers of CD34(+) cells for cancer immunotherapy. |
Mendeley readers
The data shown below were compiled from readership statistics for 186 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Sweden | 2 | 1% |
| Netherlands | 1 | <1% |
| Germany | 1 | <1% |
| India | 1 | <1% |
| United States | 1 | <1% |
| Unknown | 180 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 42 | 23% |
| Student > Ph. D. Student | 27 | 15% |
| Student > Master | 22 | 12% |
| Student > Bachelor | 19 | 10% |
| Student > Doctoral Student | 13 | 7% |
| Other | 23 | 12% |
| Unknown | 40 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 45 | 24% |
| Immunology and Microbiology | 29 | 16% |
| Biochemistry, Genetics and Molecular Biology | 29 | 16% |
| Medicine and Dentistry | 26 | 14% |
| Engineering | 6 | 3% |
| Other | 7 | 4% |
| Unknown | 44 | 24% |
Attention Score in Context
This research output has an Altmetric Attention Score of 25. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 March 2024.
All research outputs
#1,501,551
of 25,366,663 outputs
Outputs from PLOS ONE
#18,706
of 220,130 outputs
Outputs of similar age
#7,515
of 192,274 outputs
Outputs of similar age from PLOS ONE
#85
of 691 outputs
Altmetric has tracked 25,366,663 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 94th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 220,130 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.7. This one has done particularly well, scoring higher than 91% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 192,274 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 96% of its contemporaries.
We're also able to compare this research output to 691 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 87% of its contemporaries.