You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
Mendeley readers
| Chapter title |
Cellular Localization of Engineered Human LINE-1 RNA and Proteins.
|
|---|---|
| Chapter number | 18 |
| Book title |
Transposons and Retrotransposons
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3372-3_18 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3370-9, 978-1-4939-3372-3
|
| Authors |
Aurélien J. Doucet, Eugénia Basyuk, Nicolas Gilbert |
| Editors |
Jose L. Garcia-Pérez |
| Abstract |
The human LINE-1 retrotransposon has the ability to mobilize into a new genomic location through an intracellular replication cycle. Immunofluorescence and in situ hybridization experiments have been developed to detect subcellular localization of retrotransposition intermediates (i.e., ORF1p, ORF2p, and L1 mRNA). Currently, these protocols are also used to validate the interaction between retrotransposition complex components and potential cellular partners involved in L1 replication. Here, we describe in details methods for the identification of LINE-1 proteins and/or RNA in cells transfected with vectors expressing engineered human LINE-1 elements. |
Mendeley readers
The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Hungary | 1 | 11% |
| Unknown | 8 | 89% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 33% |
| Student > Master | 2 | 22% |
| Researcher | 2 | 22% |
| Professor | 1 | 11% |
| Professor > Associate Professor | 1 | 11% |
| Other | 0 | 0% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 44% |
| Agricultural and Biological Sciences | 2 | 22% |
| Economics, Econometrics and Finance | 1 | 11% |
| Medicine and Dentistry | 1 | 11% |
| Neuroscience | 1 | 11% |
| Other | 0 | 0% |