Title |
BB0172, a Borrelia burgdorferi Outer Membrane Protein That Binds Integrin α3β1
|
---|---|
Published in |
Journal of Bacteriology, May 2013
|
DOI | 10.1128/jb.00187-13 |
Pubmed ID | |
Authors |
Elaine Wood, Silvia Tamborero, Ismael Mingarro, Maria D. Esteve-Gassent |
Abstract |
Lyme disease is a multisystemic disorder caused by Borrelia burgdorferi infection. Upon infection, some B. burgdorferi genes are upregulated, including members of the microbial surface components recognizing adhesive matrix molecule (MSCRAMM) protein family, which facilitate B. burgdorferi adherence to extracellular matrix components of the host. Comparative genome analysis has revealed a new family of B. burgdorferi proteins containing the von Willebrand factor A (vWFA) domain. In the present study, we characterized the expression and membrane association of the vWFA domain-containing protein BB0172 by using in vitro transcription/translation systems in the presence of microsomal membranes and with detergent phase separation assays. Our results showed evidence of BB0172 localization in the outer membrane, the orientation of the vWFA domain to the extracellular environment, and its function as a metal ion-dependent integrin-binding protein. This is the first report of a borrelial adhesin with a metal ion-dependent adhesion site (MIDAS) motif that is similar to those observed in eukaryotic integrins and has a similar function. |
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United States | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Russia | 2 | 5% |
United States | 1 | 2% |
Poland | 1 | 2% |
Unknown | 39 | 91% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 8 | 19% |
Researcher | 6 | 14% |
Student > Bachelor | 3 | 7% |
Student > Doctoral Student | 3 | 7% |
Professor | 3 | 7% |
Other | 8 | 19% |
Unknown | 12 | 28% |
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Biochemistry, Genetics and Molecular Biology | 7 | 16% |
Immunology and Microbiology | 6 | 14% |
Medicine and Dentistry | 4 | 9% |
Unspecified | 1 | 2% |
Other | 4 | 9% |
Unknown | 12 | 28% |