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Shotgun Proteomics

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Cover of 'Shotgun Proteomics'

Table of Contents

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    Book Overview
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    Chapter 1 Survey of shotgun proteomics.
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    Chapter 2 LC-MALDI-TOF/TOF for Shotgun Proteomics.
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    Chapter 3 Fully automatable multidimensional reversed-phase liquid chromatography with online tandem mass spectrometry.
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    Chapter 4 GeLC-MS/MS Analysis of Complex Protein Mixtures.
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    Chapter 5 IPG Strip-Based Peptide Fractionation for Shotgun Proteomics.
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    Chapter 6 SILAC Yeast: From Labeling to Comprehensive Proteome Quantification.
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    Chapter 7 Analysis of proteome dynamics in mice by isotopic labeling.
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    Chapter 8 Stable Isotope Labeling in Mammals (SILAM).
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    Chapter 9 Analysis of individual protein turnover in live animals on a proteome-wide scale.
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    Chapter 10 Determining Protein Subcellular Localization in Mammalian Cell Culture with Biochemical Fractionation and iTRAQ 8-Plex Quantification.
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    Chapter 11 Brain Quantitative Proteomics Combining GeLC-MS and Isotope-Coded Protein Labeling (ICPL)
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    Chapter 12 Employing TMT Quantification in a Shotgun-MS Platform.
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    Chapter 13 Employing TMT Quantification in Shotgun-MS Proteomic Analysis: A Focus on Skeletal Muscle.
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    Chapter 14 Spectral counting label-free proteomics.
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    Chapter 15 Quantification of Proteins by Label-Free LC-MS(E.).
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    Chapter 16 Bioinformatics for proteomics: opportunities at the interface between the scientists, their experiments, and the community.
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    Chapter 17 Identification of DNA Damage Checkpoint-Dependent Protein Interactions in Saccharomyces cerevisiae Using Quantitative Mass Spectrometry.
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    Chapter 18 Application of shotgun proteomics for discovery-driven protein-protein interaction.
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    Chapter 19 Mapping protein complexes using covalently linked antibodies and isobaric mass tags.
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    Chapter 20 Biomarker verification using selected reaction monitoring and shotgun proteomics.
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    Chapter 21 Use of Universal Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)-Based Selected Reaction Monitoring (SRM) Approach for Verification of Breast Cancer-Related Protein Markers.
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    Chapter 22 The Secretome Analysis by High-Throughput Proteomics and Multiple Reaction Monitoring (MRM).
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    Chapter 23 Preparation of heteroelement-incorporated and stable isotope-labeled protein standards for quantitative proteomics.
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    Chapter 24 One-source peptide/phosphopeptide ratio standards for accurate and site-specific determination of the degree of phosphorylation.
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    Chapter 25 Quantitative glycoproteomics for N-glycoproteome profiling.
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    Chapter 26 A practical recipe to survey phosphoproteomes.
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    Chapter 27 Quantitation of the Phosphoproteome Using the Library-Assisted eXtracted Ion Chromatogram (LAXIC) Strategy.
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    Chapter 28 Fast, efficient, and quality-controlled phosphopeptide enrichment from minute sample amounts using titanium dioxide.
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    Chapter 29 Quantifying small molecule-induced changes in cellular protein expression and posttranslational modifications using isobaric mass tags.
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    Chapter 30 Analysis of protein structure by cross-linking combined with mass spectrometry.
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    Chapter 31 Top-down proteomics by means of orbitrap mass spectrometry.
Attention for Chapter 29: Quantifying small molecule-induced changes in cellular protein expression and posttranslational modifications using isobaric mass tags.
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Chapter title
Quantifying small molecule-induced changes in cellular protein expression and posttranslational modifications using isobaric mass tags.
Chapter number 29
Book title
Shotgun Proteomics
Published in
Methods in molecular biology, April 2014
DOI 10.1007/978-1-4939-0685-7_29
Pubmed ID
Book ISBNs
978-1-4939-0684-0, 978-1-4939-0685-7
Authors

Isabelle Becher, Maria Fälth Savitski, Marcus Bantscheff

Editors

Daniel Martins-de-Souza

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 2 100%

Demographic breakdown

Readers by professional status Count As %
Unspecified 1 50%
Student > Ph. D. Student 1 50%
Readers by discipline Count As %
Unspecified 1 50%
Medicine and Dentistry 1 50%