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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Doxycycline Alters Metabolism and Proliferation of Human Cell Lines
|
|---|---|
| Published in |
PLOS ONE, May 2013
|
| DOI | 10.1371/journal.pone.0064561 |
| Pubmed ID | |
| Authors |
Ethan Ahler, William J. Sullivan, Ashley Cass, Daniel Braas, Autumn G. York, Steven J. Bensinger, Thomas G. Graeber, Heather R. Christofk |
| Abstract |
The tetracycline antibiotics are widely used in biomedical research as mediators of inducible gene expression systems. Despite many known effects of tetracyclines on mammalian cells-including inhibition of the mitochondrial ribosome-there have been few reports on potential off-target effects at concentrations commonly used in inducible systems. Here, we report that in human cell lines, commonly used concentrations of doxycycline change gene expression patterns and concomitantly shift metabolism towards a more glycolytic phenotype, evidenced by increased lactate secretion and reduced oxygen consumption. We also show that these concentrations are sufficient to slow proliferation. These findings suggest that researchers using doxycycline in inducible expression systems should design appropriate controls to account for potential confounding effects of the drug on cellular metabolism. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 50% |
| Scientists | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 373 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Switzerland | 1 | <1% |
| Netherlands | 1 | <1% |
| France | 1 | <1% |
| United Kingdom | 1 | <1% |
| Mexico | 1 | <1% |
| Unknown | 368 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 111 | 30% |
| Student > Master | 53 | 14% |
| Student > Bachelor | 51 | 14% |
| Researcher | 38 | 10% |
| Student > Doctoral Student | 17 | 5% |
| Other | 31 | 8% |
| Unknown | 72 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 111 | 30% |
| Agricultural and Biological Sciences | 101 | 27% |
| Medicine and Dentistry | 29 | 8% |
| Immunology and Microbiology | 12 | 3% |
| Chemistry | 8 | 2% |
| Other | 32 | 9% |
| Unknown | 80 | 21% |
Attention Score in Context
This research output has an Altmetric Attention Score of 15. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 September 2022.
All research outputs
#2,367,311
of 24,780,938 outputs
Outputs from PLOS ONE
#29,229
of 214,469 outputs
Outputs of similar age
#19,622
of 199,154 outputs
Outputs of similar age from PLOS ONE
#699
of 4,753 outputs
Altmetric has tracked 24,780,938 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 90th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 214,469 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.6. This one has done well, scoring higher than 86% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 199,154 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 90% of its contemporaries.
We're also able to compare this research output to 4,753 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 85% of its contemporaries.