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Microchip Diagnostics

Overview of attention for book
Cover of 'Microchip Diagnostics'

Table of Contents

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    Book Overview
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    Chapter 1 Microfluidics-Enabled Diagnostic Systems: Markets, Challenges, and Examples.
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    Chapter 2 Capillary-Driven Microfluidic Chips for Miniaturized Immunoassays: Efficient Fabrication and Sealing of Chips Using a "Chip-Olate" Process.
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    Chapter 3 Capillary-Driven Microfluidic Chips for Miniaturized Immunoassays: Patterning Capture Antibodies Using Microcontact Printing and Dry-Film Resists.
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    Chapter 4 Fabrication and Evaluation of Microfluidic Immunoassay Devices with Antibody-Immobilized Microbeads Retained in Porous Hydrogel Micropillars.
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    Chapter 5 Using Electrophoretic Immunoassay to Monitor Hormone Secretion.
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    Chapter 6 On-Chip Magnetic Particle-Based Immunoassays Using Multilaminar Flow for Clinical Diagnostics.
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    Chapter 7 Digital Microfluidics Assisted Sealing of Individual Magnetic Particles in Femtoliter-Sized Reaction Wells for Single-Molecule Detection.
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    Chapter 8 Microfluidic DNA Stretching Device for Single-Molecule Diagnostics.
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    Chapter 9 Droplet Microfluidic and Magnetic Particles Platform for Cancer Typing.
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    Chapter 10 SlipChip Device for Digital Nucleic Acid Amplification.
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    Chapter 11 Multiplex Detection of KRAS Mutations Using Passive Droplet Fusion.
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    Chapter 12 Droplet-Based Microfluidics Digital PCR for the Detection of KRAS Mutations.
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    Chapter 13 Negative Enrichment of Circulating Tumor Cells in Blood Using a Microfluidic Chip.
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    Chapter 14 Microfluidic-Based Bacteria Isolation from Whole Blood for Diagnostics of Blood Stream Infection.
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    Chapter 15 Microfluidic Platform for Parallel Single Cell Analysis for Diagnostic Applications.
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    Chapter 16 FISH-in-CHIPS: A Microfluidic Platform for Molecular Typing of Cancer Cells.
Attention for Chapter 6: On-Chip Magnetic Particle-Based Immunoassays Using Multilaminar Flow for Clinical Diagnostics.
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Chapter title
On-Chip Magnetic Particle-Based Immunoassays Using Multilaminar Flow for Clinical Diagnostics.
Chapter number 6
Book title
Microchip Diagnostics
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6734-6_6
Pubmed ID
Book ISBNs
978-1-4939-6732-2, 978-1-4939-6734-6
Authors

Mark D. Tarn, Nicole Pamme

Editors

Valérie Taly, Jean-Louis Viovy, Stéphanie Descroix

Abstract

Magnetic particles have become popular in recent years for immunoassays due to their high surface-to-volume ratio and the ease of their manipulation. However, such assays also require multiple reaction and washing steps that are both time-consuming and manually laborious. Here, we describe a setup and methodology for performing rapid immunoassays on magnetic particles in continuous flow via their deflection through multiple laminar flow streams of reagents and washing solutions. In particular, we focus on the use of the microfluidic platform for a C-reactive protein (CRP) sandwich immunoassay in less than 60 s.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 24 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 6 25%
Student > Master 3 13%
Researcher 3 13%
Student > Bachelor 2 8%
Professor 2 8%
Other 4 17%
Unknown 4 17%
Readers by discipline Count As %
Engineering 5 21%
Chemistry 4 17%
Biochemistry, Genetics and Molecular Biology 3 13%
Arts and Humanities 2 8%
Chemical Engineering 2 8%
Other 6 25%
Unknown 2 8%