Title |
Neuronal sFlt1 and Vegfaa determine venous sprouting and spinal cord vascularization
|
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Published in |
Nature Communications, January 2017
|
DOI | 10.1038/ncomms13991 |
Pubmed ID | |
Authors |
Raphael Wild, Alina Klems, Masanari Takamiya, Yuya Hayashi, Uwe Strähle, Koji Ando, Naoki Mochizuki, Andreas van Impel, Stefan Schulte-Merker, Janna Krueger, Laetitia Preau, Ferdinand le Noble |
Abstract |
Formation of organ-specific vasculatures requires cross-talk between developing tissue and specialized endothelial cells. Here we show how developing zebrafish spinal cord neurons coordinate vessel growth through balancing of neuron-derived Vegfaa, with neuronal sFlt1 restricting Vegfaa-Kdrl mediated angiogenesis at the neurovascular interface. Neuron-specific loss of flt1 or increased neuronal vegfaa expression promotes angiogenesis and peri-neural tube vascular network formation. Combining loss of neuronal flt1 with gain of vegfaa promotes sprout invasion into the neural tube. On loss of neuronal flt1, ectopic sprouts emanate from veins involving special angiogenic cell behaviours including nuclear positioning and a molecular signature distinct from primary arterial or secondary venous sprouting. Manipulation of arteriovenous identity or Notch signalling established that ectopic sprouting in flt1 mutants requires venous endothelium. Conceptually, our data suggest that spinal cord vascularization proceeds from veins involving two-tiered regulation of neuronal sFlt1 and Vegfaa via a novel sprouting mode. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 4 | 27% |
France | 2 | 13% |
Mexico | 1 | 7% |
United Kingdom | 1 | 7% |
Unknown | 7 | 47% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 12 | 80% |
Scientists | 2 | 13% |
Science communicators (journalists, bloggers, editors) | 1 | 7% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Portugal | 1 | <1% |
Unknown | 140 | 99% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 34 | 24% |
Researcher | 22 | 16% |
Student > Master | 20 | 14% |
Student > Bachelor | 16 | 11% |
Professor > Associate Professor | 4 | 3% |
Other | 13 | 9% |
Unknown | 32 | 23% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 53 | 38% |
Agricultural and Biological Sciences | 28 | 20% |
Neuroscience | 11 | 8% |
Medicine and Dentistry | 7 | 5% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 1% |
Other | 7 | 5% |
Unknown | 33 | 23% |