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Molecular characterization of a novel orthomyxovirus from rainbow and steelhead trout (Oncorhynchus mykiss)

Overview of attention for article published in Virus Research, February 2017
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Mentioned by

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2 tweeters

Citations

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10 Dimensions

Readers on

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35 Mendeley
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Title
Molecular characterization of a novel orthomyxovirus from rainbow and steelhead trout (Oncorhynchus mykiss)
Published in
Virus Research, February 2017
DOI 10.1016/j.virusres.2017.01.005
Pubmed ID
Authors

William N. Batts, Scott E. LaPatra, Ryan Katona, Eric Leis, Terry Fei Fan Ng, Marine S.O. Brieuc, Rachel B. Breyta, Maureen K. Purcell, Carla M. Conway, Thomas B. Waltzek, Eric Delwart, James R. Winton

Abstract

A novel virus, rainbow trout orthomyxovirus (RbtOV), was isolated in 1997 and again in 2000 from commercially-reared rainbow trout (Oncorhynchus mykiss) in Idaho, USA. The virus grew optimally in the CHSE-214 cell line at 15°C producing a diffuse cytopathic effect; however, juvenile rainbow trout exposed to cell culture-grown virus showed no mortality or gross pathology. Electron microscopy of preparations from infected cell cultures revealed the presence of typical orthomyxovirus particles. The complete genome of RbtOV is comprised of eight linear segments of single-stranded, negative-sense RNA having highly conserved 5' and 3'-terminal nucleotide sequences. Another virus isolated in 2014 from steelhead trout (also O. mykiss) in Wisconsin, USA, and designated SttOV was found to have eight genome segments with high amino acid sequence identities (89-99%) to the corresponding genes of RbtOV, suggesting these new viruses are isolates of the same virus species and may be more widespread than currently realized. The new isolates had the same genome segment order and the closest pairwise amino acid sequence identities of 16-42% with Infectious salmon anemia virus (ISAV), the type species and currently only member of the genus Isavirus in the family Orthomyxoviridae. However, pairwise comparisons of the predicted amino acid sequences of the 10 RbtOV and SttOV proteins with orthologs from representatives of the established orthomyxoviral genera and a phylogenetic analysis using the PB1 protein showed that while RbtOV and SttOV clustered most closely with ISAV, they diverged sufficiently to merit consideration as representatives of a novel genus. A set of PCR primers was designed using conserved regions of the PB1 gene to produce amplicons that may be sequenced for identification of similar fish orthomyxoviruses in the future.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 35 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 35 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 10 29%
Student > Ph. D. Student 6 17%
Student > Master 5 14%
Other 3 9%
Lecturer 1 3%
Other 3 9%
Unknown 7 20%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 7 20%
Veterinary Science and Veterinary Medicine 6 17%
Agricultural and Biological Sciences 5 14%
Environmental Science 2 6%
Immunology and Microbiology 2 6%
Other 4 11%
Unknown 9 26%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 December 2017.
All research outputs
#7,068,218
of 12,298,022 outputs
Outputs from Virus Research
#1,194
of 2,001 outputs
Outputs of similar age
#157,489
of 331,621 outputs
Outputs of similar age from Virus Research
#8
of 47 outputs
Altmetric has tracked 12,298,022 research outputs across all sources so far. This one is in the 40th percentile – i.e., 40% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,001 research outputs from this source. They receive a mean Attention Score of 3.1. This one is in the 38th percentile – i.e., 38% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 331,621 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 49th percentile – i.e., 49% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 47 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 82% of its contemporaries.