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Reverse Genetics of RNA Viruses

Overview of attention for book
Attention for Chapter 6: Rescue of Infectious Salmon Anemia Virus (ISAV) from Cloned cDNA.
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  • Above-average Attention Score compared to outputs of the same age (60th percentile)
  • High Attention Score compared to outputs of the same age and source (83rd percentile)

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Chapter title
Rescue of Infectious Salmon Anemia Virus (ISAV) from Cloned cDNA.
Chapter number 6
Book title
Reverse Genetics of RNA Viruses
Published in
Methods in molecular biology, January 2024
DOI 10.1007/978-1-0716-3533-9_6
Pubmed ID
Book ISBNs
978-1-07-163532-2, 978-1-07-163533-9
Authors

Toro-Ascuy, Daniela, Cárdenas, Matías, Vásquez-Martínez, Yesseny, Cortez-San Martín, Marcelo

Abstract

The piscine orthomyxovirus called infectious salmon anemia virus (ISAV) is one of the most important emerging pathogens affecting the salmon industry worldwide. The first reverse genetics system for ISAV, which allows the generation of recombinant ISA virus (rISAV), is an important tool for the characterization and study of this virus. The plasmid-based reverse genetics system for ISAV includes the use of a novel fish promoter, the Atlantic salmon internal transcribed spacer region 1 (ITS-1). The salmon, viral, and mammalian genetic elements included in the pSS-URG vectors allow the expression of the eight viral RNA segments. In addition to four cytomegalovirus (CMV)-based vectors that express the four proteins of the ISAV ribonucleoprotein complex, the eight pSS-URG vectors allowed the generation of infectious rISAV in salmon cells.

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Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 December 2023.
All research outputs
#14,936,362
of 25,010,497 outputs
Outputs from Methods in molecular biology
#4,048
of 14,079 outputs
Outputs of similar age
#43,502
of 115,721 outputs
Outputs of similar age from Methods in molecular biology
#31
of 190 outputs
Altmetric has tracked 25,010,497 research outputs across all sources so far. This one is in the 38th percentile – i.e., 38% of other outputs scored the same or lower than it.
So far Altmetric has tracked 14,079 research outputs from this source. They receive a mean Attention Score of 3.5. This one has gotten more attention than average, scoring higher than 69% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 115,721 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 60% of its contemporaries.
We're also able to compare this research output to 190 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 83% of its contemporaries.