Chapter title |
Rescue of Infectious Salmon Anemia Virus (ISAV) from Cloned cDNA.
|
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Chapter number | 6 |
Book title |
Reverse Genetics of RNA Viruses
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Published in |
Methods in molecular biology, January 2024
|
DOI | 10.1007/978-1-0716-3533-9_6 |
Pubmed ID | |
Book ISBNs |
978-1-07-163532-2, 978-1-07-163533-9
|
Authors |
Toro-Ascuy, Daniela, Cárdenas, Matías, Vásquez-Martínez, Yesseny, Cortez-San Martín, Marcelo |
Abstract |
The piscine orthomyxovirus called infectious salmon anemia virus (ISAV) is one of the most important emerging pathogens affecting the salmon industry worldwide. The first reverse genetics system for ISAV, which allows the generation of recombinant ISA virus (rISAV), is an important tool for the characterization and study of this virus. The plasmid-based reverse genetics system for ISAV includes the use of a novel fish promoter, the Atlantic salmon internal transcribed spacer region 1 (ITS-1). The salmon, viral, and mammalian genetic elements included in the pSS-URG vectors allow the expression of the eight viral RNA segments. In addition to four cytomegalovirus (CMV)-based vectors that express the four proteins of the ISAV ribonucleoprotein complex, the eight pSS-URG vectors allowed the generation of infectious rISAV in salmon cells. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |