Title |
Proposal of a quantitative PCR-based protocol for an optimal Pseudomonas aeruginosa detection in patients with cystic fibrosis
|
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Published in |
BMC Microbiology, June 2013
|
DOI | 10.1186/1471-2180-13-143 |
Pubmed ID | |
Authors |
Florence Le Gall, Rozenn Le Berre, Sylvain Rosec, Jeanne Hardy, Stéphanie Gouriou, Sylvie Boisramé-Gastrin, Sophie Vallet, Gilles Rault, Christopher Payan, Geneviève Héry-Arnaud |
Abstract |
The lung of patients with cystic fibrosis (CF) is particularly sensitive to Pseudomonas aeruginosa. This bacterium plays an important role in the poor outcome of CF patients. During the disease progress, first acquisition of P. aeruginosa is the key-step in the management of CF patients. Quantitative PCR (qPCR) offers an opportunity to detect earlier the first acquisition of P. aeruginosa by CF patients. Given the lack of a validated protocol, our goal was to find an optimal molecular protocol for detection of P. aeruginosa in CF patients. |
X Demographics
Geographical breakdown
Country | Count | As % |
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France | 3 | 50% |
United Kingdom | 2 | 33% |
Unknown | 1 | 17% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 4 | 67% |
Scientists | 1 | 17% |
Science communicators (journalists, bloggers, editors) | 1 | 17% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 1% |
Portugal | 1 | 1% |
Unknown | 73 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 24 | 32% |
Researcher | 15 | 20% |
Student > Bachelor | 7 | 9% |
Student > Master | 6 | 8% |
Student > Doctoral Student | 5 | 7% |
Other | 7 | 9% |
Unknown | 11 | 15% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 23 | 31% |
Biochemistry, Genetics and Molecular Biology | 12 | 16% |
Medicine and Dentistry | 10 | 13% |
Immunology and Microbiology | 9 | 12% |
Engineering | 3 | 4% |
Other | 7 | 9% |
Unknown | 11 | 15% |