| Chapter title |
Microscopy techniques to examine DNA replication in fission yeast.
|
|---|---|
| Chapter number | 26 |
| Book title |
DNA Replication
|
| Published in |
Methods in molecular biology, July 2009
|
| DOI | 10.1007/978-1-60327-815-7_26 |
| Pubmed ID | |
| Book ISBNs |
978-1-60327-814-0, 978-1-60327-815-7
|
| Authors |
Green MD, Sabatinos SA, Forsburg SL, Green, Marc D., Sabatinos, Sarah A., Forsburg, Susan L., Marc D. Green, Sarah A. Sabatinos, Susan L. Forsburg |
| Abstract |
Temporal and spatial visualization of replication proteins and associated structures within the narrow confines of a yeast nucleus is technically challenging. Choosing the appropriate method depends upon the parameters of the experiment, the nature of the molecules to be observed, and the hypothesis to be tested. In this chapter, we review three broad types of visualization: whole cell fluorescence or immunofluorescence, which is useful for questions of timing and chromatin association; nuclear spreads, which provide greater resolution within the chromatin for colocalization and region-specific effects; and chromatin fibers, which allow observation of labeled proteins and newly synthesized DNA on a linear chromosome. We discuss applications of these protocols and some considerations for choosing methods and fluorophores. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 1 | 50% |
| Unknown | 1 | 50% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 1 | 50% |
| Unknown | 1 | 50% |