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| Chapter title |
Immunohistochemical Detection of the Autophagy Markers LC3 and p62/SQSTM1 in Formalin-Fixed and Paraffin-Embedded Tissue
|
|---|---|
| Chapter number | 13 |
| Book title |
Histochemistry of Single Molecules
|
| Published in |
Methods in molecular biology, February 2017
|
| DOI | 10.1007/978-1-4939-6788-9_13 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6787-2, 978-1-4939-6788-9
|
| Authors |
Sabina Berezowska, José A. Galván |
| Editors |
Carlo Pellicciari, Marco Biggiogera |
| Abstract |
Autophagy is a highly conserved cellular mechanism of "self digestion," ensuring cellular homeostasis, and playing a role in many diseases including cancer. As a stress response mechanism, it may also be involved in cellular response to therapy.LC3 and Sequestosome 1 (p62/SQSTM1) are among the most widely used markers to monitor autophagy, and can be visualized in formalin-fixed and paraffin-embedded tissue by immunohistochemistry. Here we describe a validated staining protocol using an automated staining system available in many routine pathology laboratories, enabling high-throughput staining under standardized conditions. |
Mendeley readers
The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 20 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 5 | 25% |
| Student > Postgraduate | 4 | 20% |
| Researcher | 2 | 10% |
| Student > Bachelor | 2 | 10% |
| Other | 1 | 5% |
| Other | 3 | 15% |
| Unknown | 3 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 6 | 30% |
| Biochemistry, Genetics and Molecular Biology | 5 | 25% |
| Agricultural and Biological Sciences | 3 | 15% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 10% |
| Immunology and Microbiology | 1 | 5% |
| Other | 0 | 0% |
| Unknown | 3 | 15% |