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Protein Expression in Mammalian Cells

Overview of attention for book
Cover of 'Protein Expression in Mammalian Cells'

Table of Contents

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    Book Overview
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    Chapter 1 Why Proteins in Mammalian Cells?
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    Chapter 2 Large-Scale Transfection of Mammalian Cells
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    Chapter 3 Selection of High Expressing Mammalian Cells by Surface Display of Reporters
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    Chapter 4 Expression of a Secreted Protein in Mammalian Cells Using Baculovirus Particles
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    Chapter 5 Transfection of Difficult-to-Transfect Primary Mammalian Cells
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    Chapter 6 Stable Protein Expression in Mammalian Cells Using Baculoviruses
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    Chapter 7 Protein Expression in Mammalian Cells
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    Chapter 8 Protein Expression in Mammalian Cells
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    Chapter 9 Post-transcriptional Regulatory Elements for Enhancing Transient Gene Expression Levels in Mammalian Cells
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    Chapter 10 Protein Expression in Mammalian Cells
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    Chapter 11 Generation of High-Expressing Cells by Methotrexate Amplification of Destabilized Dihydrofolate Reductase Selection Marker
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    Chapter 12 Protein Expression in Mammalian Cells
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    Chapter 13 Identification and characterization of protein glycosylation using specific endo- and exoglycosidases.
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    Chapter 14 Protein Expression in Mammalian Cells
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    Chapter 15 Methods for Constructing Clones for Protein Expression in Mammalian Cells
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    Chapter 16 Optimizing Transient Recombinant Protein Expression in Mammalian Cells
Attention for Chapter 13: Identification and characterization of protein glycosylation using specific endo- and exoglycosidases.
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Chapter title
Identification and characterization of protein glycosylation using specific endo- and exoglycosidases.
Chapter number 13
Book title
Protein Expression in Mammalian Cells
Published in
Methods in molecular biology, September 2011
DOI 10.1007/978-1-61779-352-3_13
Pubmed ID
Book ISBNs
978-1-61779-351-6, 978-1-61779-352-3
Authors

Paula Magnelli, Alicia Bielik, Ellen Guthrie, Magnelli, Paula, Bielik, Alicia, Guthrie, Ellen

Editors

James L Hartley

Abstract

Enzymatic deglycosylation followed by SDS-PAGE is a valuable method to detect glycan modifications on protein samples. Specific glycosidases were used to remove sugars from glycoproteins in a controlled fashion leaving the protein core intact; the resulting change in molecular weight could be detected as shifts in gel mobility. Alternatively, glycan-sensitive reagents were used to visualize the intensity of glycoprotein bands before and after enzyme treatment. The ease of use of these techniques, which require only basic laboratory instrumentation and reagents, makes them the methodology of choice for initial glycobiology studies. These protocols are also well suited to screen for optimal expression conditions, since multiple glycoprotein samples can be processed at once.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 34 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 34 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 8 24%
Student > Ph. D. Student 8 24%
Student > Bachelor 4 12%
Student > Master 4 12%
Student > Doctoral Student 2 6%
Other 3 9%
Unknown 5 15%
Readers by discipline Count As %
Agricultural and Biological Sciences 12 35%
Biochemistry, Genetics and Molecular Biology 6 18%
Chemistry 5 15%
Engineering 2 6%
Medicine and Dentistry 2 6%
Other 2 6%
Unknown 5 15%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 November 2011.
All research outputs
#11,728,619
of 14,788,347 outputs
Outputs from Methods in molecular biology
#4,485
of 8,789 outputs
Outputs of similar age
#153,957
of 208,749 outputs
Outputs of similar age from Methods in molecular biology
#253
of 420 outputs
Altmetric has tracked 14,788,347 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 8,789 research outputs from this source. They receive a mean Attention Score of 2.5. This one is in the 29th percentile – i.e., 29% of its peers scored the same or lower than it.
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We're also able to compare this research output to 420 others from the same source and published within six weeks on either side of this one. This one is in the 14th percentile – i.e., 14% of its contemporaries scored the same or lower than it.