Title |
Description of a double centrifugation tube method for concentrating canine platelets
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Published in |
BMC Veterinary Research, July 2013
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DOI | 10.1186/1746-6148-9-146 |
Pubmed ID | |
Authors |
Anna Perazzi, Roberto Busetto, Tiziana Martinello, Michele Drigo, Daniela Pasotto, Francesco Cian, Marco Patruno, Ilaria Iacopetti |
Abstract |
To evaluate the efficiency of platelet-rich plasma preparations by means of a double centrifugation tube method to obtain platelet-rich canine plasma at a concentration at least 4 times higher than the baseline value and a concentration of white blood cells not exceeding twice the reference range. A complete blood count was carried out for each sample and each concentrate. Whole blood samples were collected from 12 clinically healthy dogs (consenting blood donors). Blood was processed by a double centrifugation tube method to obtain platelet concentrates, which were then analyzed by a flow cytometry haematology system for haemogram. Platelet concentration and white blood cell count were determined in all samples. |
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