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Purification and characterization of a novel ubiquitin-like antitumour protein with hemagglutinating and deoxyribonuclease activities from the edible mushroom Ramaria botrytis

Overview of attention for article published in AMB Express, February 2017
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1 tweeter

Citations

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17 Dimensions

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Title
Purification and characterization of a novel ubiquitin-like antitumour protein with hemagglutinating and deoxyribonuclease activities from the edible mushroom Ramaria botrytis
Published in
AMB Express, February 2017
DOI 10.1186/s13568-017-0346-9
Pubmed ID
Authors

Rong Zhou, Ya-Jie Han, Min-Hui Zhang, Ke-Ren Zhang, Tzi Bun Ng, Fang Liu

Abstract

A novel ubiquitin-like antitumour protein (RBUP) was isolated from fruiting bodies of the edible mushroom Ramaria botrytis. The protein was isolated with a purification protocol involving ion exchange chromatography on DEAE-Sepharose fast flow and gel filtration on Sephadex G-75. SDS-PAGE, Native-PAGE and ultracentrifugation analysis disclosed that RBUP was a monomeric protein with a molecular weight of 18.5 kDa. ESI-MS/MS demonstrated that it shared 69% amino acid sequence similarity with Coprinellus congregates ubiquitin (gi|136667). The protein exhibiting strong anticancer activity towards A549 cells. Analysis by employing AO/EB staining and Annexin V-FITC/PI detection indicated that the cytotoxic effect of RBUP was mediated through induction of apoptosis. Furthermore, RBUP displayed hemagglutinating and deoxyribonuclease activities. A temperature of 40 °C and pH of 7.0 were required for optimal DNase activity. Therefore, it was estimated that RBUP exerted its antitumour effect by inducing apoptosis, and its hemagglutinating and DNase activities were also thought to participate in this effect. These results demonstrated that RBUP was a multifunctional protein with potential medicinal applications.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Japan 1 6%
Unknown 16 94%

Demographic breakdown

Readers by professional status Count As %
Professor 2 12%
Other 2 12%
Student > Ph. D. Student 2 12%
Librarian 1 6%
Student > Bachelor 1 6%
Other 4 24%
Unknown 5 29%
Readers by discipline Count As %
Agricultural and Biological Sciences 4 24%
Chemistry 2 12%
Biochemistry, Genetics and Molecular Biology 2 12%
Unspecified 1 6%
Medicine and Dentistry 1 6%
Other 1 6%
Unknown 6 35%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 February 2017.
All research outputs
#4,949,585
of 9,105,319 outputs
Outputs from AMB Express
#254
of 583 outputs
Outputs of similar age
#149,356
of 255,347 outputs
Outputs of similar age from AMB Express
#18
of 54 outputs
Altmetric has tracked 9,105,319 research outputs across all sources so far. This one is in the 27th percentile – i.e., 27% of other outputs scored the same or lower than it.
So far Altmetric has tracked 583 research outputs from this source. They receive a mean Attention Score of 2.2. This one is in the 7th percentile – i.e., 7% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 255,347 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 32nd percentile – i.e., 32% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 54 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.