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Factors influencing detection of eDNA from a stream-dwelling amphibian

Overview of attention for article published in Molecular Ecology Resources, September 2013
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  • High Attention Score compared to outputs of the same age and source (80th percentile)

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1 tweeter
1 patent


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618 Mendeley
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Factors influencing detection of eDNA from a stream-dwelling amphibian
Published in
Molecular Ecology Resources, September 2013
DOI 10.1111/1755-0998.12159
Pubmed ID

David S. Pilliod, Caren S. Goldberg, Robert S. Arkle, Lisette P. Waits


Environmental DNA (eDNA) methods for detecting and estimating abundance of aquatic species are emerging rapidly, but little is known about how processes such as secretion rate, environmental degradation, and time since colonization or extirpation from a given site affect eDNA measurements. Using stream-dwelling salamanders and quantitative PCR (qPCR) analysis, we conducted three experiments to assess eDNA: (i) production rate; (ii) persistence time under different temperature and light conditions; and (iii) detectability and concentration through time following experimental introduction and removal of salamanders into previously unoccupied streams. We found that 44-50 g individuals held in aquaria produced 77 ng eDNA/h for 2 h, after which production either slowed considerably or began to equilibrate with degradation. eDNA in both full-sun and shaded treatments degraded exponentially to <1% of the original concentration after 3 days. eDNA was no longer detectable in full-sun samples after 8 days, whereas eDNA was detected in 20% of shaded samples after 11 days and 100% of refrigerated control samples after 18 days. When translocated into unoccupied streams, salamanders were detectable after 6 h, but only when densities were relatively high (0.2481 individuals/m(2) ) and when samples were collected within 5 m of the animals. Concentrations of eDNA detected were very low and increased steadily from 6-24 h after introduction, reaching 0.0022 ng/L. Within 1 h of removing salamanders from the stream, eDNA was no longer detectable. These results suggest that eDNA detectability and concentration depend on production rates of individuals, environmental conditions, density of animals, and their residence time.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 618 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 5 <1%
United Kingdom 3 <1%
Brazil 3 <1%
Netherlands 2 <1%
Australia 1 <1%
France 1 <1%
Portugal 1 <1%
Canada 1 <1%
Germany 1 <1%
Other 2 <1%
Unknown 598 97%

Demographic breakdown

Readers by professional status Count As %
Student > Master 123 20%
Researcher 120 19%
Student > Ph. D. Student 104 17%
Student > Bachelor 89 14%
Other 33 5%
Other 66 11%
Unknown 83 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 269 44%
Environmental Science 131 21%
Biochemistry, Genetics and Molecular Biology 80 13%
Earth and Planetary Sciences 8 1%
Engineering 7 1%
Other 23 4%
Unknown 100 16%

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 October 2022.
All research outputs
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Outputs from Molecular Ecology Resources
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Outputs of similar age
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Outputs of similar age from Molecular Ecology Resources
of 20 outputs
Altmetric has tracked 22,962,258 research outputs across all sources so far. This one has received more attention than most of these and is in the 68th percentile.
So far Altmetric has tracked 1,636 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 11.3. This one is in the 43rd percentile – i.e., 43% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 197,582 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 67% of its contemporaries.
We're also able to compare this research output to 20 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 80% of its contemporaries.