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Factors influencing detection of eDNA from a stream-dwelling amphibian

Overview of attention for article published in Molecular Ecology Resources, September 2013
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Title
Factors influencing detection of eDNA from a stream-dwelling amphibian
Published in
Molecular Ecology Resources, September 2013
DOI 10.1111/1755-0998.12159
Pubmed ID
Authors

David S. Pilliod, Caren S. Goldberg, Robert S. Arkle, Lisette P. Waits

Abstract

Environmental DNA (eDNA) methods for detecting and estimating abundance of aquatic species are emerging rapidly, but little is known about how processes such as secretion rate, environmental degradation, and time since colonization or extirpation from a given site affect eDNA measurements. Using stream-dwelling salamanders and quantitative PCR (qPCR) analysis, we conducted three experiments to assess eDNA: (i) production rate; (ii) persistence time under different temperature and light conditions; and (iii) detectability and concentration through time following experimental introduction and removal of salamanders into previously unoccupied streams. We found that 44-50 g individuals held in aquaria produced 77 ng eDNA/h for 2 h, after which production either slowed considerably or began to equilibrate with degradation. eDNA in both full-sun and shaded treatments degraded exponentially to <1% of the original concentration after 3 days. eDNA was no longer detectable in full-sun samples after 8 days, whereas eDNA was detected in 20% of shaded samples after 11 days and 100% of refrigerated control samples after 18 days. When translocated into unoccupied streams, salamanders were detectable after 6 h, but only when densities were relatively high (0.2481 individuals/m(2) ) and when samples were collected within 5 m of the animals. Concentrations of eDNA detected were very low and increased steadily from 6-24 h after introduction, reaching 0.0022 ng/L. Within 1 h of removing salamanders from the stream, eDNA was no longer detectable. These results suggest that eDNA detectability and concentration depend on production rates of individuals, environmental conditions, density of animals, and their residence time.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 419 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 6 1%
United Kingdom 3 <1%
Brazil 3 <1%
Netherlands 2 <1%
Spain 1 <1%
Australia 1 <1%
France 1 <1%
Portugal 1 <1%
Germany 1 <1%
Other 2 <1%
Unknown 398 95%

Demographic breakdown

Readers by professional status Count As %
Researcher 94 22%
Student > Master 83 20%
Student > Ph. D. Student 72 17%
Student > Bachelor 61 15%
Unspecified 32 8%
Other 77 18%
Readers by discipline Count As %
Agricultural and Biological Sciences 222 53%
Environmental Science 94 22%
Biochemistry, Genetics and Molecular Biology 47 11%
Unspecified 42 10%
Earth and Planetary Sciences 5 1%
Other 9 2%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 August 2013.
All research outputs
#10,036,014
of 12,543,614 outputs
Outputs from Molecular Ecology Resources
#794
of 895 outputs
Outputs of similar age
#105,237
of 153,234 outputs
Outputs of similar age from Molecular Ecology Resources
#15
of 21 outputs
Altmetric has tracked 12,543,614 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 895 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 9.0. This one is in the 4th percentile – i.e., 4% of its peers scored the same or lower than it.
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We're also able to compare this research output to 21 others from the same source and published within six weeks on either side of this one. This one is in the 4th percentile – i.e., 4% of its contemporaries scored the same or lower than it.