Chapter title |
Antibody Validation by Immunoprecipitation Followed by Mass Spectrometry Analysis
|
---|---|
Chapter number | 10 |
Book title |
Synthetic Antibodies
|
Published in |
Methods in molecular biology, March 2017
|
DOI | 10.1007/978-1-4939-6857-2_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6855-8, 978-1-4939-6857-2
|
Authors |
Helena Persson, Charlotta Preger, Edyta Marcon, Johan Lengqvist, Susanne Gräslund |
Editors |
Thomas Tiller |
Abstract |
We describe a mass spectrometry-based approach for validation of antibody specificity. This method allows validation of antibodies or antibody fragments, against their endogenous targets. It can assess if the antibody is able to bind to its native antigen in cell lysates among thousands of other proteins, DNA, RNA, and other cellular components. In addition, it identifies other proteins the antibody is able to immunoprecipitate allowing for the assessment of antibody specificity and selectivity. This method is easily scalable, adaptable to different cell lines and conditions and has been shown to be reproducible between multiple laboratories. |
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