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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Standardized and flexible eight colour flow cytometry panels harmonized between different laboratories to study human NK cell phenotype and function
|
|---|---|
| Published in |
Scientific Reports, March 2017
|
| DOI | 10.1038/srep43873 |
| Pubmed ID | |
| Authors |
John P. Veluchamy, María Delso-Vallejo, Nina Kok, Fenna Bohme, Ruth Seggewiss-Bernhardt, Hans J. van der Vliet, Tanja D. de Gruijl, Volker Huppert, Jan Spanholtz |
| Abstract |
Advancements in multi-colour fluorescence activated cell sorting (FACS) panel warrant harmonized procedures to obtain comparable data between various laboratories. The intensifying clinical exploration of Natural Killer (NK) cell-based immunotherapy demands standardized and harmonized NK cell FACS panels and acquisition protocols. Eight colour FACS panels were designed to study human NK cell phenotype and function within peripheral blood mononuclear cells (PBMC). The panels were designed around fixed backbone markers and channels, covering antigens for non-NK lineage exclusion (CD3, TCRγδ, CD19, CD14, SYTOX(®) Blue) and NK cell selection (CD45, CD56, CD16), complemented with variable drop-in markers/channels to study NK cell phenotype (NKG2A, NKG2C, NKG2D and KIR2D) or NK cell function and activation (CD25, NKp44 and CD107a). Harmonized FACS set-up and data analysis for three different flow cytometers has been established, leading to highly comparable and reproducible data sets using the same PBMC reference samples (n = 6). Further studies of NK cells in fresh or cryopreserved PBMC samples (n = 12) confirmed that freezing and thawing of PBMC samples did not significantly affect NK phenotype or function. In conclusion, our data demonstrate that cryopreserved PBMC samples analysed by standardized FACS panels and harmonized analysis protocols will generate highly reliable data sets for multi-center clinical trials under validated conditions. |
X Demographics
The data shown below were collected from the profiles of 10 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Canada | 2 | 20% |
| Russia | 1 | 10% |
| Germany | 1 | 10% |
| United States | 1 | 10% |
| Unknown | 5 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 8 | 80% |
| Science communicators (journalists, bloggers, editors) | 1 | 10% |
| Scientists | 1 | 10% |
Mendeley readers
The data shown below were compiled from readership statistics for 139 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| India | 1 | <1% |
| Germany | 1 | <1% |
| France | 1 | <1% |
| Unknown | 136 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 30 | 22% |
| Student > Ph. D. Student | 21 | 15% |
| Student > Bachelor | 14 | 10% |
| Student > Master | 13 | 9% |
| Other | 7 | 5% |
| Other | 16 | 12% |
| Unknown | 38 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 25 | 18% |
| Immunology and Microbiology | 23 | 17% |
| Agricultural and Biological Sciences | 16 | 12% |
| Medicine and Dentistry | 16 | 12% |
| Nursing and Health Professions | 3 | 2% |
| Other | 18 | 13% |
| Unknown | 38 | 27% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 March 2017.
All research outputs
#5,729,908
of 23,590,588 outputs
Outputs from Scientific Reports
#39,109
of 127,467 outputs
Outputs of similar age
#89,649
of 308,845 outputs
Outputs of similar age from Scientific Reports
#1,489
of 4,594 outputs
Altmetric has tracked 23,590,588 research outputs across all sources so far. Compared to these this one has done well and is in the 75th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 127,467 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 18.4. This one has gotten more attention than average, scoring higher than 69% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 308,845 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 70% of its contemporaries.
We're also able to compare this research output to 4,594 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 67% of its contemporaries.