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Biomolecular Characterization of Diazotrophs Isolated from the Tropical Soil in Malaysia

Overview of attention for article published in International Journal of Molecular Sciences, August 2013
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Title
Biomolecular Characterization of Diazotrophs Isolated from the Tropical Soil in Malaysia
Published in
International Journal of Molecular Sciences, August 2013
DOI 10.3390/ijms140917812
Pubmed ID
Authors

Umme Naher, Radziah Othman, Mohammad Latif, Qurban Panhwar, Puteri Amaddin, Zulkifli Shamsuddin

Abstract

This study was conducted to evaluate selected biomolecular characteristics of rice root-associated diazotrophs isolated from the Tanjong Karang rice irrigation project area of Malaysia. Soil and rice plant samples were collected from seven soil series belonging to order Inceptisol (USDA soil taxonomy). A total of 38 diazotrophs were isolated using a nitrogen-free medium. The biochemical properties of the isolated bacteria, such as nitrogenase activity, indoleacetic acid (IAA) production and sugar utilization, were measured. According to a cluster analysis of Jaccard's similarity coefficients, the genetic similarities among the isolated diazotrophs ranged from 10% to 100%. A dendogram constructed using the unweighted pair-group method with arithmetic mean (UPGMA) showed that the isolated diazotrophs clustered into 12 groups. The genomic DNA rep-PCR data were subjected to a principal component analysis, and the first four principal components (PC) accounted for 52.46% of the total variation among the 38 diazotrophs. The 10 diazotrophs that tested highly positive in the acetylene reduction assay (ARA) were identified as Bacillus spp. (9 diazotrophs) and Burkholderia sp. (Sb16) using the partial 16S rRNA gene sequence analysis. In the analysis of the biochemical characteristics, three principal components were accounted for approximately 85% of the total variation among the identified diazotrophs. The examination of root colonization using scanning electron microscopy (SEM) and transmission electron microscopy (TEM) proved that two of the isolated diazotrophs (Sb16 and Sb26) were able to colonize the surface and interior of rice roots and fixed 22%-24% of the total tissue nitrogen from the atmosphere. In general, the tropical soils (Inceptisols) of the Tanjong Karang rice irrigation project area in Malaysia harbor a diverse group of diazotrophs that exhibit a large variation of biomolecular characteristics.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 2 6%
Austria 1 3%
France 1 3%
Australia 1 3%
United Kingdom 1 3%
Colombia 1 3%
Unknown 25 78%

Demographic breakdown

Readers by professional status Count As %
Student > Master 9 28%
Student > Ph. D. Student 9 28%
Researcher 5 16%
Lecturer 2 6%
Professor > Associate Professor 2 6%
Other 2 6%
Unknown 3 9%
Readers by discipline Count As %
Agricultural and Biological Sciences 20 63%
Computer Science 3 9%
Environmental Science 2 6%
Materials Science 2 6%
Earth and Planetary Sciences 1 3%
Other 0 0%
Unknown 4 13%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 August 2013.
All research outputs
#11,167,920
of 12,552,783 outputs
Outputs from International Journal of Molecular Sciences
#6,810
of 8,833 outputs
Outputs of similar age
#131,047
of 155,212 outputs
Outputs of similar age from International Journal of Molecular Sciences
#44
of 49 outputs
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