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Biomolecular Characterization of Diazotrophs Isolated from the Tropical Soil in Malaysia

Overview of attention for article published in International Journal of Molecular Sciences, August 2013
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Title
Biomolecular Characterization of Diazotrophs Isolated from the Tropical Soil in Malaysia
Published in
International Journal of Molecular Sciences, August 2013
DOI 10.3390/ijms140917812
Pubmed ID
Authors

Umme Aminun Naher, Radziah Othman, Mohammad Abdul Latif, Qurban Ali Panhwar, Puteri Aminatulhawa Megat Amaddin, Zulkifli H Shamsuddin

Abstract

This study was conducted to evaluate selected biomolecular characteristics of rice root-associated diazotrophs isolated from the Tanjong Karang rice irrigation project area of Malaysia. Soil and rice plant samples were collected from seven soil series belonging to order Inceptisol (USDA soil taxonomy). A total of 38 diazotrophs were isolated using a nitrogen-free medium. The biochemical properties of the isolated bacteria, such as nitrogenase activity, indoleacetic acid (IAA) production and sugar utilization, were measured. According to a cluster analysis of Jaccard's similarity coefficients, the genetic similarities among the isolated diazotrophs ranged from 10% to 100%. A dendogram constructed using the unweighted pair-group method with arithmetic mean (UPGMA) showed that the isolated diazotrophs clustered into 12 groups. The genomic DNA rep-PCR data were subjected to a principal component analysis, and the first four principal components (PC) accounted for 52.46% of the total variation among the 38 diazotrophs. The 10 diazotrophs that tested highly positive in the acetylene reduction assay (ARA) were identified as Bacillus spp. (9 diazotrophs) and Burkholderia sp. (Sb16) using the partial 16S rRNA gene sequence analysis. In the analysis of the biochemical characteristics, three principal components were accounted for approximately 85% of the total variation among the identified diazotrophs. The examination of root colonization using scanning electron microscopy (SEM) and transmission electron microscopy (TEM) proved that two of the isolated diazotrophs (Sb16 and Sb26) were able to colonize the surface and interior of rice roots and fixed 22%-24% of the total tissue nitrogen from the atmosphere. In general, the tropical soils (Inceptisols) of the Tanjong Karang rice irrigation project area in Malaysia harbor a diverse group of diazotrophs that exhibit a large variation of biomolecular characteristics.

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Geographical breakdown

Country Count As %
United States 2 5%
France 1 2%
Colombia 1 2%
United Kingdom 1 2%
Austria 1 2%
Unknown 38 86%

Demographic breakdown

Readers by professional status Count As %
Student > Master 11 25%
Student > Ph. D. Student 9 20%
Researcher 5 11%
Student > Bachelor 2 5%
Lecturer 2 5%
Other 5 11%
Unknown 10 23%
Readers by discipline Count As %
Agricultural and Biological Sciences 22 50%
Computer Science 3 7%
Environmental Science 2 5%
Biochemistry, Genetics and Molecular Biology 2 5%
Materials Science 2 5%
Other 2 5%
Unknown 11 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 August 2013.
All research outputs
#20,657,128
of 25,374,917 outputs
Outputs from International Journal of Molecular Sciences
#31,896
of 44,336 outputs
Outputs of similar age
#160,639
of 211,843 outputs
Outputs of similar age from International Journal of Molecular Sciences
#88
of 137 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. This one is in the 10th percentile – i.e., 10% of other outputs scored the same or lower than it.
So far Altmetric has tracked 44,336 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.0. This one is in the 16th percentile – i.e., 16% of its peers scored the same or lower than it.
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We're also able to compare this research output to 137 others from the same source and published within six weeks on either side of this one. This one is in the 8th percentile – i.e., 8% of its contemporaries scored the same or lower than it.