Chapter title |
Isolation of Mouse Stromal Vascular Cells for Monolayer Culture
|
---|---|
Chapter number | 2 |
Book title |
Thermogenic Fat
|
Published in |
Methods in molecular biology, February 2017
|
DOI | 10.1007/978-1-4939-6820-6_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6819-0, 978-1-4939-6820-6
|
Authors |
Longhua Liu, Louise D. Zheng, Sarah R. Donnelly, Margo P. Emont, Jun Wu, Zhiyong Cheng |
Editors |
Jun Wu |
Abstract |
Positive energy balance contributes to adipose tissue expansion and dysfunction, which accounts largely for obesity and related metabolic disorders. Thermogenic fat can dissipate energy, activation or induction of which may promote energy balance and address the pressing health issues. Recent studies have shown that stromal vascular fraction (SVF) from white adipose tissue (WAT) can develop both white and brown-like adipocyte phenotypes, thus serving as a unique model to study adipogenesis and thermogenesis. Here, we describe a protocol for effective isolation of mouse SVF from WAT, induction of differentiation, and detection of adipogenesis. Success tips for isolation and culture of SVF are also discussed. |
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Geographical breakdown
Country | Count | As % |
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Unknown | 9 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Professor | 3 | 33% |
Student > Master | 2 | 22% |
Student > Doctoral Student | 1 | 11% |
Lecturer | 1 | 11% |
Professor > Associate Professor | 1 | 11% |
Other | 0 | 0% |
Unknown | 1 | 11% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 1 | 11% |
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Unknown | 3 | 33% |