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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Heritable and Precise Zebrafish Genome Editing Using a CRISPR-Cas System
|
|---|---|
| Published in |
PLOS ONE, July 2013
|
| DOI | 10.1371/journal.pone.0068708 |
| Pubmed ID | |
| Authors |
Woong Y. Hwang, Yanfang Fu, Deepak Reyon, Morgan L. Maeder, Prakriti Kaini, Jeffry D. Sander, J. Keith Joung, Randall T. Peterson, Jing-Ruey Joanna Yeh |
| Abstract |
We have previously reported a simple and customizable CRISPR (clustered regularly interspaced short palindromic repeats) RNA-guided Cas9 nuclease (RGN) system that can be used to efficiently and robustly introduce somatic indel mutations in endogenous zebrafish genes. Here we demonstrate that RGN-induced mutations are heritable, with efficiencies of germline transmission reaching as high as 100%. In addition, we extend the power of the RGN system by showing that these nucleases can be used with single-stranded oligodeoxynucleotides (ssODNs) to create precise intended sequence modifications, including single nucleotide substitutions. Finally, we describe and validate simple strategies that improve the targeting range of RGNs from 1 in every 128 basepairs (bps) of random DNA sequence to 1 in every 8 bps. Together, these advances expand the utility of the CRISPR-Cas system in the zebrafish beyond somatic indel formation to heritable and precise genome modifications. |
X Demographics
The data shown below were collected from the profiles of 6 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 1 | 17% |
| Canada | 1 | 17% |
| Ireland | 1 | 17% |
| United States | 1 | 17% |
| Unknown | 2 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 4 | 67% |
| Scientists | 2 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 683 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 7 | 1% |
| United Kingdom | 4 | <1% |
| Japan | 3 | <1% |
| China | 3 | <1% |
| France | 2 | <1% |
| Denmark | 2 | <1% |
| Germany | 2 | <1% |
| Portugal | 2 | <1% |
| Finland | 1 | <1% |
| Other | 6 | <1% |
| Unknown | 651 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 158 | 23% |
| Researcher | 127 | 19% |
| Student > Bachelor | 87 | 13% |
| Student > Master | 85 | 12% |
| Student > Doctoral Student | 32 | 5% |
| Other | 94 | 14% |
| Unknown | 100 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 320 | 47% |
| Biochemistry, Genetics and Molecular Biology | 159 | 23% |
| Medicine and Dentistry | 31 | 5% |
| Neuroscience | 24 | 4% |
| Engineering | 8 | 1% |
| Other | 35 | 5% |
| Unknown | 106 | 16% |
Attention Score in Context
This research output has an Altmetric Attention Score of 38. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 March 2024.
All research outputs
#956,180
of 23,504,998 outputs
Outputs from PLOS ONE
#12,848
of 201,347 outputs
Outputs of similar age
#8,206
of 195,791 outputs
Outputs of similar age from PLOS ONE
#365
of 4,771 outputs
Altmetric has tracked 23,504,998 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 95th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 201,347 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.3. This one has done particularly well, scoring higher than 93% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 195,791 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 95% of its contemporaries.
We're also able to compare this research output to 4,771 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 92% of its contemporaries.