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Prion protein protocols

Overview of attention for book
Cover of 'Prion protein protocols'

Table of Contents

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    Book Overview
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    Chapter 1 Cell Culture Models to Unravel Prion Protein Function and Aberrancies in Prion Diseases
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    Chapter 2 Prion Protein Protocols
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    Chapter 3 Immunodetection of PrP Sc Using Western and Slot Blotting Techniques
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    Chapter 4 Assaying prions in cell culture: the standard scrapie cell assay (SSCA) and the scrapie cell assay in end point format (SCEPA).
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    Chapter 5 Generation of Cell Lines Propagating Infectious Prions and the Isolation and Characterization of Cell-derived Exosomes
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    Chapter 6 Neurotoxicity of Prion Peptides on Cultured Cerebellar Neurons
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    Chapter 7 Understanding the Nature of Prion Diseases Using Cell-free Assays
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    Chapter 8 Methods for Conversion of Prion Protein into Amyloid Fibrils
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    Chapter 9 Amplification of Purified Prions In Vitro
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    Chapter 10 Expression and Purification of Full-Length Recombinant PrP of High Purity
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    Chapter 11 Analysis of PrP Conformation Using Circular Dichroism
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    Chapter 12 Effect of Copper on the De Novo Generation of Prion Protein Expressed in Pichia pastoris
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    Chapter 13 Biophysical Investigations of the Prion Protein Using Electron Paramagnetic Resonance
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    Chapter 14 Molecular Diagnosis of Human Prion Disease
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    Chapter 15 Analysis of Endogenous PrPC Processing in Neuronal and Non-neuronal Cell Lines
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    Chapter 16 Molecular Typing of PrPres in Human Sporadic CJD Brain Tissue
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    Chapter 17 Transgenic Mouse Models of Prion Diseases
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    Chapter 18 Quantitative Bioassay of Surface-bound Prion Infectivity
Attention for Chapter 5: Generation of Cell Lines Propagating Infectious Prions and the Isolation and Characterization of Cell-derived Exosomes
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Chapter title
Generation of Cell Lines Propagating Infectious Prions and the Isolation and Characterization of Cell-derived Exosomes
Chapter number 5
Book title
Prion Protein Protocols
Published in
Methods in molecular biology, June 2008
DOI 10.1007/978-1-59745-234-2_5
Pubmed ID
Book ISBNs
978-1-58829-897-3, 978-1-59745-234-2
Authors

Vella, Laura J., Hill, Andrew F., Laura J. Vella, Andrew F. Hill

Abstract

Prion-propagating cell lines are an efficient and useful means for studying the cellular and molecular mechanisms implicated in prion disease. Use of cell-based models has lead to the finding that prion protein (PrP(C)) and PrP(Sc) are released from cells in association with exosomes. Furthermore, exosomes have been shown to act as vehicles for infectivity, transferring PrP(Sc) between cell lines and providing a mechanism for prion spread between tissues. As a role for exosomes in prion disease is emerging, this chapter outlines a method for the generation of prion-infected cell lines and the isolation and characterization of PrP(C)- and PrP(Sc)-containing exosomes.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 36 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Denmark 1 3%
Argentina 1 3%
Unknown 34 94%

Demographic breakdown

Readers by professional status Count As %
Researcher 8 22%
Student > Ph. D. Student 7 19%
Professor 5 14%
Professor > Associate Professor 5 14%
Student > Doctoral Student 2 6%
Other 6 17%
Unknown 3 8%
Readers by discipline Count As %
Agricultural and Biological Sciences 16 44%
Biochemistry, Genetics and Molecular Biology 7 19%
Medicine and Dentistry 6 17%
Immunology and Microbiology 2 6%
Neuroscience 2 6%
Other 0 0%
Unknown 3 8%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 December 2012.
All research outputs
#6,193,055
of 7,185,574 outputs
Outputs from Methods in molecular biology
#3,433
of 4,944 outputs
Outputs of similar age
#242,661
of 291,020 outputs
Outputs of similar age from Methods in molecular biology
#180
of 234 outputs
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