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Mammosphere Formation in Breast Carcinoma Cell Lines Depends upon Expression of E-cadherin

Overview of attention for article published in PLOS ONE, October 2013
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  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (76th percentile)
  • Good Attention Score compared to outputs of the same age and source (70th percentile)

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8 X users

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Title
Mammosphere Formation in Breast Carcinoma Cell Lines Depends upon Expression of E-cadherin
Published in
PLOS ONE, October 2013
DOI 10.1371/journal.pone.0077281
Pubmed ID
Authors

Juan Manuel Iglesias, Izaskun Beloqui, Francisco Garcia-Garcia, Olatz Leis, Alejandro Vazquez-Martin, Arrate Eguiara, Silvia Cufi, Andres Pavon, Javier A. Menendez, Joaquin Dopazo, Angel G. Martin

Abstract

Tumors are heterogeneous at the cellular level where the ability to maintain tumor growth resides in discrete cell populations. Floating sphere-forming assays are broadly used to test stem cell activity in tissues, tumors and cell lines. Spheroids are originated from a small population of cells with stem cell features able to grow in suspension culture and behaving as tumorigenic in mice. We tested the ability of eleven common breast cancer cell lines representing the major breast cancer subtypes to grow as mammospheres, measuring the ability to maintain cell viability upon serial non-adherent passage. Only MCF7, T47D, BT474, MDA-MB-436 and JIMT1 were successfully propagated as long-term mammosphere cultures, measured as the increase in the number of viable cells upon serial non-adherent passages. Other cell lines tested (SKBR3, MDA-MB-231, MDA-MB-468 and MDA-MB-435) formed cell clumps that can be disaggregated mechanically, but cell viability drops dramatically on their second passage. HCC1937 and HCC1569 cells formed typical mammospheres, although they could not be propagated as long-term mammosphere cultures. All the sphere forming lines but MDA-MB-436 express E-cadherin on their surface. Knock down of E-cadherin expression in MCF-7 cells abrogated its ability to grow as mammospheres, while re-expression of E-cadherin in SKBR3 cells allow them to form mammospheres. Therefore, the mammosphere assay is suitable to reveal stem like features in breast cancer cell lines that express E-cadherin.

X Demographics

X Demographics

The data shown below were collected from the profiles of 8 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 370 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 3 <1%
Spain 3 <1%
Netherlands 2 <1%
India 2 <1%
Canada 1 <1%
United Kingdom 1 <1%
Mexico 1 <1%
Unknown 357 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 86 23%
Researcher 61 16%
Student > Master 55 15%
Student > Bachelor 42 11%
Student > Doctoral Student 23 6%
Other 49 13%
Unknown 54 15%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 113 31%
Agricultural and Biological Sciences 113 31%
Medicine and Dentistry 25 7%
Engineering 13 4%
Pharmacology, Toxicology and Pharmaceutical Science 11 3%
Other 26 7%
Unknown 69 19%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 June 2017.
All research outputs
#5,951,181
of 24,335,016 outputs
Outputs from PLOS ONE
#80,165
of 209,795 outputs
Outputs of similar age
#49,883
of 212,721 outputs
Outputs of similar age from PLOS ONE
#1,479
of 5,001 outputs
Altmetric has tracked 24,335,016 research outputs across all sources so far. Compared to these this one has done well and is in the 75th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 209,795 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.6. This one has gotten more attention than average, scoring higher than 61% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 212,721 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 76% of its contemporaries.
We're also able to compare this research output to 5,001 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 70% of its contemporaries.