Title |
A quick and low-cost PCR-based assay for Candidaspp. identification in positive blood culture bottles
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Published in |
BMC Infectious Diseases, October 2013
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DOI | 10.1186/1471-2334-13-467 |
Pubmed ID | |
Authors |
Hemílio Xafranski, Analy SA Melo, Antonia M Machado, Marcelo RS Briones, Arnaldo L Colombo |
Abstract |
Differences in the susceptibility of Candida species to antifungal drugs make identification to the species level important for clinical management of candidemia. Molecular tests are not yet standardized or available in most clinical laboratories, although such tests can reduce the time required for species identification, as compared to the conventional culture-based methods. To decrease laboratory costs and improve diagnostic accuracy, different molecular methods have been proposed, including DNA extraction protocols to produce pure DNA free of PCR inhibitors. The objective of this study was to validate a new format of molecular method, based on the internal transcribed spacer (ITS) of the rDNA gene amplification followed by sequencing, to identify common and cryptic Candida species causing candidemia by analyzing DNA in blood culture bottles positive for yeasts. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
India | 1 | 2% |
Brazil | 1 | 2% |
Unknown | 39 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 8 | 20% |
Researcher | 6 | 15% |
Student > Master | 5 | 12% |
Student > Bachelor | 3 | 7% |
Other | 3 | 7% |
Other | 10 | 24% |
Unknown | 6 | 15% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 16 | 39% |
Medicine and Dentistry | 9 | 22% |
Biochemistry, Genetics and Molecular Biology | 5 | 12% |
Immunology and Microbiology | 1 | 2% |
Nursing and Health Professions | 1 | 2% |
Other | 2 | 5% |
Unknown | 7 | 17% |