Title |
Plasticity of primary microglia on micropatterned geometries and spontaneous long-distance migration in microfluidic channels
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Published in |
BMC Neuroscience, October 2013
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DOI | 10.1186/1471-2202-14-121 |
Pubmed ID | |
Authors |
Susanna Amadio, Adele De Ninno, Cinzia Montilli, Luca Businaro, Annamaria Gerardino, Cinzia Volonté |
Abstract |
Microglia possess an elevated grade of plasticity, undergoing several structural changes based on their location and state of activation. The first step towards the comprehension of microglia's biology and functional responses to an extremely mutable extracellular milieu, consists in discriminating the morphological features acquired by cells maintained in vitro under diverse environmental conditions. Previous work described neither primary microglia grown on artificially patterned environments which impose physical cues and constraints, nor long distance migration of microglia in vitro. To this aim, the present work exploits artificial bio-mimetic microstructured substrates with pillar-shaped or line-grating geometries fabricated on poly(dimethylsiloxane) by soft lithography, in addition to microfluidic devices, and highlights some morphological/functional characteristics of microglia which were underestimated or unknown so far. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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France | 1 | 2% |
Unknown | 50 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 14 | 27% |
Student > Master | 10 | 20% |
Student > Doctoral Student | 9 | 18% |
Student > Ph. D. Student | 8 | 16% |
Student > Bachelor | 2 | 4% |
Other | 4 | 8% |
Unknown | 4 | 8% |
Readers by discipline | Count | As % |
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Neuroscience | 9 | 18% |
Agricultural and Biological Sciences | 9 | 18% |
Biochemistry, Genetics and Molecular Biology | 8 | 16% |
Engineering | 7 | 14% |
Medicine and Dentistry | 4 | 8% |
Other | 5 | 10% |
Unknown | 9 | 18% |