Chapter title |
Studying coronavirus-host protein interactions.
|
---|---|
Chapter number | 17 |
Book title |
Coronaviruses
|
Published in |
Methods in molecular biology, February 2015
|
DOI | 10.1007/978-1-4939-2438-7_17 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2437-0, 978-1-4939-2438-7
|
Authors |
Chee-Hing Yang, Hui-Chun Li, Cheng-Huei Hung, Shih-Yen Lo, Yang, Chee-Hing, Li, Hui-Chun, Hung, Cheng-Huei, Lo, Shih-Yen |
Editors |
Helena Jane Maier, Erica Bickerton, Paul Britton |
Abstract |
To understand the molecular mechanisms of viral replication and pathogenesis, it is necessary to establish the virus-host protein interaction networks. The yeast two-hybrid system is a powerful proteomic approach to study protein-protein interactions. After the identification of specific cellular factors interacting with the target viral protein using the yeast two-hybrid screening system, co-immunoprecipitation and confocal microscopy analyses are often used to verify the virus-host protein interactions in cells. Identification of the cellular factors required for viral survival or eliminating virus infected cells could help scientists develop more effective antiviral drugs. Here we summarize a standard protocol used in our lab to study the coronavirus-host protein interactions, including yeast two-hybrid screening, co-immunoprecipitation, and immunofluorescence microscopy analyses. |
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