Chapter title |
Targeting Promoter-Associated RNAs by siRNAs
|
---|---|
Chapter number | 11 |
Book title |
Promoter Associated RNA
|
Published in |
Methods in molecular biology, March 2017
|
DOI | 10.1007/978-1-4939-6716-2_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6714-8, 978-1-4939-6716-2
|
Authors |
Napoli, Sara, Sara Napoli |
Editors |
Sara Napoli |
Abstract |
Promoter-associated RNAs (paRNA) are long noncoding RNAs transcribed in sense or antisense direction in correspondence of promoters of other genes. Their expression influences promoter activity by means of specific regulatory function. PaRNA can act as a scaffold for proteins involved in processes regulating gene expression, as chromatin remodeling or transcription. RNA-induced transcriptional silencing (RITS) is the mechanism of transcriptional interference achieved by siRNA directed against chromatin-associated RNAs, as paRNAs. In this chapter, it is described how to detect interaction between siRNA and paRNA in cell nuclei and investigate siRNA capability to destabilize paRNA-protein complex. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 2 | 67% |
France | 1 | 33% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 67% |
Scientists | 1 | 33% |