Title |
Role of a GenoType MTBDRplus line probe assay in early detection of multidrug-resistant tuberculosis at a Brazilian reference center
|
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Published in |
Brazilian Journal of Medical and Biological Research, August 2015
|
DOI | 10.1590/1414-431x20154458 |
Pubmed ID | |
Authors |
C.S. Feliciano, M.M.P. Nascimento, L.M.P. Anselmo, R.H.C. Pocente, F. Bellissimo-Rodrigues, V.R. Bollela |
Abstract |
Resistance to Mycobacterium tuberculosis is a reality worldwide, and its diagnosis continues to be difficult and time consuming. To face this challenge, the World Health Organization has recommended the use of rapid molecular tests. We evaluated the routine use (once a week) of a line probe assay (Genotype MTBDRplus) for early diagnosis of resistance and for assessment of the main related risk factors over 2 years. A total of 170 samples were tested: 15 (8.8%) were resistant, and multidrug resistance was detected in 10 (5.9%). The sensitivity profile took 3 weeks (2 weeks for culture and 1 week for rapid testing). Previous treatment for tuberculosis and the persistence of positive acid-fast smears after 4 months of supervised treatment were the major risk factors observed. The use of molecular tests enabled early diagnosis of drug-resistant bacilli and led to appropriate treatment of the disease. This information has the potential to interrupt the transmission chain of resistant M. tuberculosis. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Brazil | 1 | 1% |
Unknown | 70 | 99% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 16 | 23% |
Researcher | 11 | 15% |
Student > Bachelor | 6 | 8% |
Student > Doctoral Student | 5 | 7% |
Other | 5 | 7% |
Other | 14 | 20% |
Unknown | 14 | 20% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 23 | 32% |
Immunology and Microbiology | 7 | 10% |
Agricultural and Biological Sciences | 6 | 8% |
Biochemistry, Genetics and Molecular Biology | 4 | 6% |
Nursing and Health Professions | 3 | 4% |
Other | 10 | 14% |
Unknown | 18 | 25% |