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Exosomal transfer of tumor-associated macrophage-derived miR-21 confers cisplatin resistance in gastric cancer cells

Overview of attention for article published in Journal of Experimental & Clinical Cancer Research, April 2017
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (54th percentile)

Mentioned by

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6 tweeters
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1 Facebook page

Citations

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350 Dimensions

Readers on

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151 Mendeley
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Title
Exosomal transfer of tumor-associated macrophage-derived miR-21 confers cisplatin resistance in gastric cancer cells
Published in
Journal of Experimental & Clinical Cancer Research, April 2017
DOI 10.1186/s13046-017-0528-y
Pubmed ID
Authors

Peiming Zheng, Lei Chen, Xiangliang Yuan, Qin Luo, Yi Liu, Guohua Xie, Yanhui Ma, Lisong Shen

Abstract

Cisplatin-based chemotherapy is frequently used to treat advanced gastric cancer (GC). However, the resistance often occurs with the mechanisms being not well understood. Recently, emerging evidence indicates that tumor-associated macrophages (TAMs) play an important role in chemoresistance of cancer. As the important mediators in intercellular communications, exosomes secreted by host cells mediate the exchange of genetic materials and proteins to be involved in tumor aggressiveness. The aim of the study was to investigate whether exosomes derived from TAMs mediate cisplatin resistance in gastric cancer. M2 polarized macrophages were obtained from mouse bone marrow or human PBMCs stimulated with IL-4 and IL-13. Exosomes isolated from M2 macrophages culture medium were characterized, and miRNA expression profiles of M2 derived exosomes (M2-exos) were analyzed using miRNA microarray. In vitro cell coculture was further conducted to investigate M2-exos mediated crosstalk between TAMs and tumor cells. Moreover, the in vivo experiments were performed using a subcutaneous transplantation tumor model in athymic nude mice. In this study, we showed that M2 polarized macrophages promoted cisplatin (DDP) resistance in gastric cancer cells and exosomes derived from M2 macrophages (M2-exos) are involved in mediating the resistance to DDP. Using miRNA profiles assay, we identify significantly higher levels of microRNA-21 (miR21) isomiRNAs in exosomes and cell lysate isolated from M2 polarized macrophage. Functional studies revealed that exosomal miR-21 can be directly transferred from macrophages to the gastric cancer cells, where it suppresses cell apoptosis and enhances activation of PI3K/AKT signaling pathway by down-regulation of PTEN. Our findings suggest that exosomal transfer of tumor-associated macrophages derived miR-21 confer DDP resistance in gastric cancer, and targeting exosome communication may be a promising new therapeutic strategy for gastric cancer patients.

Twitter Demographics

The data shown below were collected from the profiles of 6 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 151 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 151 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 27 18%
Student > Master 25 17%
Student > Bachelor 16 11%
Researcher 14 9%
Student > Doctoral Student 10 7%
Other 21 14%
Unknown 38 25%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 45 30%
Medicine and Dentistry 20 13%
Agricultural and Biological Sciences 13 9%
Immunology and Microbiology 7 5%
Pharmacology, Toxicology and Pharmaceutical Science 6 4%
Other 15 10%
Unknown 45 30%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 September 2017.
All research outputs
#11,936,451
of 21,326,488 outputs
Outputs from Journal of Experimental & Clinical Cancer Research
#490
of 1,877 outputs
Outputs of similar age
#128,089
of 283,382 outputs
Outputs of similar age from Journal of Experimental & Clinical Cancer Research
#1
of 1 outputs
Altmetric has tracked 21,326,488 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,877 research outputs from this source. They receive a mean Attention Score of 3.5. This one has gotten more attention than average, scoring higher than 73% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 283,382 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 54% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them