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X Demographics
Mendeley readers
Attention Score in Context
| Title |
AHT-ChIP-seq: a completely automated robotic protocol for high-throughput chromatin immunoprecipitation
|
|---|---|
| Published in |
Genome Biology, November 2013
|
| DOI | 10.1186/gb-2013-14-11-r124 |
| Pubmed ID | |
| Authors |
Sarah Aldridge, Stephen Watt, Michael A Quail, Tim Rayner, Margus Lukk, Michael F Bimson, Daniel Gaffney, Duncan T Odom |
| Abstract |
ChIP-seq is an established manually-performed method for identifying DNA-protein interactions genome-wide. Here, we describe a protocol for automated high-throughput (AHT) ChIP-seq. To demonstrate the quality of data obtained using AHT-ChIP-seq, we applied it to five proteins in mouse livers using a single 96-well plate, demonstrating an extremely high degree of qualitative and quantitative reproducibility among biological and technical replicates. We estimated the optimum and minimum recommended cell numbers required to perform AHT-ChIP-seq by running an additional plate using HepG2 and MCF7 cells. With this protocol, commercially available robotics can perform four hundred experiments in five days. |
X Demographics
The data shown below were collected from the profiles of 22 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 10 | 45% |
| United Kingdom | 3 | 14% |
| France | 1 | 5% |
| India | 1 | 5% |
| Germany | 1 | 5% |
| Unknown | 6 | 27% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 11 | 50% |
| Members of the public | 9 | 41% |
| Science communicators (journalists, bloggers, editors) | 2 | 9% |
Mendeley readers
The data shown below were compiled from readership statistics for 98 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 4 | 4% |
| United States | 4 | 4% |
| Germany | 2 | 2% |
| Spain | 2 | 2% |
| Canada | 1 | 1% |
| Italy | 1 | 1% |
| China | 1 | 1% |
| Unknown | 83 | 85% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 30 | 31% |
| Student > Ph. D. Student | 27 | 28% |
| Student > Master | 7 | 7% |
| Student > Bachelor | 5 | 5% |
| Other | 5 | 5% |
| Other | 12 | 12% |
| Unknown | 12 | 12% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 52 | 53% |
| Biochemistry, Genetics and Molecular Biology | 25 | 26% |
| Computer Science | 6 | 6% |
| Medicine and Dentistry | 2 | 2% |
| Engineering | 1 | 1% |
| Other | 0 | 0% |
| Unknown | 12 | 12% |
Attention Score in Context
This research output has an Altmetric Attention Score of 24. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 August 2022.
All research outputs
#1,601,876
of 25,371,288 outputs
Outputs from Genome Biology
#1,308
of 4,467 outputs
Outputs of similar age
#14,876
of 228,787 outputs
Outputs of similar age from Genome Biology
#17
of 56 outputs
Altmetric has tracked 25,371,288 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 93rd percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 4,467 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 27.6. This one has gotten more attention than average, scoring higher than 70% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 228,787 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 93% of its contemporaries.
We're also able to compare this research output to 56 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 69% of its contemporaries.