| Chapter title |
Recombinant Tri-Segmented Pichinde Virus as a Novel Live Viral Vaccine Platform
|
|---|---|
| Chapter number | 10 |
| Book title |
Recombinant Virus Vaccines
|
| Published in |
Methods in molecular biology, April 2017
|
| DOI | 10.1007/978-1-4939-6869-5_10 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6867-1, 978-1-4939-6869-5
|
| Authors |
Rekha Dhanwani, Hinh Ly, Yuying Liang |
| Editors |
Maureen C. Ferran, Gary R. Skuse |
| Abstract |
Pichinde virus (PICV) is a nonpathogenic arenavirus with a bi-segmented RNA genome (L and S segments) that encodes four viral genes. We have developed a reverse genetics system to generate recombinant tri-segmented PICV (rP18tri) that packages three RNA segments (L, S1, and S2) and can encode up to two foreign genes. Using influenza virus HA and NP as model antigens, we show that the rP18tri vector can induce strong humoral and cell-mediated immunity, which further increases upon a booster dose. We propose that this novel rP18tri vector can be developed into a useful vaccine platform for other antigens, particularly when strong cellular immunity and prime-boost vaccination strategy are desired. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 14 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 21% |
| Student > Bachelor | 2 | 14% |
| Other | 1 | 7% |
| Student > Master | 1 | 7% |
| Researcher | 1 | 7% |
| Other | 0 | 0% |
| Unknown | 6 | 43% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 21% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 7% |
| Computer Science | 1 | 7% |
| Immunology and Microbiology | 1 | 7% |
| Social Sciences | 1 | 7% |
| Other | 1 | 7% |
| Unknown | 6 | 43% |