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Imperfect coordination chemistry facilitates metal ion release in the Psa permease

Overview of attention for article published in Nature Chemical Biology, November 2013
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  • In the top 5% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (99th percentile)
  • High Attention Score compared to outputs of the same age and source (98th percentile)

Citations

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129 Dimensions

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115 Mendeley
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1 CiteULike
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Title
Imperfect coordination chemistry facilitates metal ion release in the Psa permease
Published in
Nature Chemical Biology, November 2013
DOI 10.1038/nchembio.1382
Pubmed ID
Authors

Rafael M Couñago, Miranda P Ween, Stephanie L Begg, Megha Bajaj, Johannes Zuegg, Megan L O'Mara, Matthew A Cooper, Alastair G McEwan, James C Paton, Bostjan Kobe, Christopher A McDevitt

Abstract

The relative stability of divalent first-row transition metal ion complexes, as defined by the Irving-Williams series, poses a fundamental chemical challenge for selectivity in bacterial metal ion acquisition. Here we show that although the substrate-binding protein of Streptococcus pneumoniae, PsaA, is finely attuned to bind its physiological substrate manganese, it can also bind a broad range of other divalent transition metal cations. By combining high-resolution structural data, metal-binding assays and mutational analyses, we show that the inability of open-state PsaA to satisfy the preferred coordination chemistry of manganese enables the protein to undergo the conformational changes required for cargo release to the Psa permease. This is specific for manganese ions, whereas zinc ions remain bound to PsaA. Collectively, these findings suggest a new ligand binding and release mechanism for PsaA and related substrate-binding proteins that facilitate specificity for divalent cations during competition from zinc ions, which are more abundant in biological systems.

X Demographics

X Demographics

The data shown below were collected from the profiles of 8 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 115 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 3 3%
India 2 2%
Korea, Republic of 1 <1%
China 1 <1%
Unknown 108 94%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 33 29%
Researcher 23 20%
Student > Bachelor 18 16%
Student > Doctoral Student 5 4%
Student > Master 5 4%
Other 13 11%
Unknown 18 16%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 31 27%
Agricultural and Biological Sciences 19 17%
Chemistry 19 17%
Immunology and Microbiology 7 6%
Engineering 6 5%
Other 14 12%
Unknown 19 17%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 167. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 August 2021.
All research outputs
#233,803
of 24,752,948 outputs
Outputs from Nature Chemical Biology
#68
of 3,291 outputs
Outputs of similar age
#1,727
of 220,102 outputs
Outputs of similar age from Nature Chemical Biology
#2
of 51 outputs
Altmetric has tracked 24,752,948 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 99th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,291 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 26.7. This one has done particularly well, scoring higher than 97% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 220,102 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 99% of its contemporaries.
We're also able to compare this research output to 51 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 98% of its contemporaries.