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Phenotypic and genotypic detection of Candida albicans and Candida dubliniensis strains isolated from oral mucosa of AIDS pediatric patients

Overview of attention for article published in Revista do Instituto de Medicina Tropical de São Paulo, January 2017
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Title
Phenotypic and genotypic detection of Candida albicans and Candida dubliniensis strains isolated from oral mucosa of AIDS pediatric patients
Published in
Revista do Instituto de Medicina Tropical de São Paulo, January 2017
DOI 10.1590/s1678-9946201759014
Pubmed ID
Authors

Harisson Oliveira Livério, Luciana da Silva Ruiz, Roseli Santos de Freitas, Angela Nishikaku, Ana Clara de Souza, Claudete Rodrigues Paula, Carina Domaneschi

Abstract

The aim of this study was to assess a collection of yeasts to verify the presence of Candida dubliniensis among strains isolated from the oral mucosa of AIDS pediatric patients which were initially characterized as Candida albicans by the traditional phenotypic method, as well as to evaluate the main phenotypic methods used in the discrimination between the two species and confirm the identification through genotypic techniques, i.e., DNA sequencing. Twenty-nine samples of C. albicans isolated from this population and kept in a fungi collection were evaluated and re-characterized. In order to differentiate the two species, phenotypic tests (Thermotolerance tests, Chromogenic medium, Staib agar, Tobacco agar, Hypertonic medium) were performed and genotypic techniques using DNA sequencing were employed for confirmation of isolated species. Susceptibility and specificity were calculated for each test. No phenotypic test alone was sufficient to provide definitive identification of C. dubliniensis or C. albicans, as opposed to results of molecular tests. After amplification and sequencing of specific regions of the 29 studied strains, 93.1% of the isolates were identified as C. albicans and 6.9% as C. dubliniensis. The Staib agar assay showed a higher susceptibility (96.3%) in comparison with other phenotypic techniques. Therefore, genotypic methods are indispensable for the conclusive identification and differentiation between these species.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 29 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 29 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 6 21%
Student > Bachelor 5 17%
Student > Doctoral Student 2 7%
Other 2 7%
Student > Ph. D. Student 2 7%
Other 4 14%
Unknown 8 28%
Readers by discipline Count As %
Medicine and Dentistry 6 21%
Immunology and Microbiology 5 17%
Biochemistry, Genetics and Molecular Biology 2 7%
Nursing and Health Professions 2 7%
Agricultural and Biological Sciences 1 3%
Other 3 10%
Unknown 10 34%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 April 2017.
All research outputs
#11,171,786
of 12,556,257 outputs
Outputs from Revista do Instituto de Medicina Tropical de São Paulo
#251
of 251 outputs
Outputs of similar age
#220,473
of 259,337 outputs
Outputs of similar age from Revista do Instituto de Medicina Tropical de São Paulo
#2
of 2 outputs
Altmetric has tracked 12,556,257 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 251 research outputs from this source. They receive a mean Attention Score of 2.9. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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