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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Generation of inner ear organoids containing functional hair cells from human pluripotent stem cells
|
|---|---|
| Published in |
Nature Biotechnology, May 2017
|
| DOI | 10.1038/nbt.3840 |
| Pubmed ID | |
| Authors |
Karl R Koehler, Jing Nie, Emma Longworth-Mills, Xiao-Ping Liu, Jiyoon Lee, Jeffrey R Holt, Eri Hashino |
| Abstract |
The derivation of human inner ear tissue from pluripotent stem cells would enable in vitro screening of drug candidates for the treatment of hearing and balance dysfunction and may provide a source of cells for cell-based therapies of the inner ear. Here we report a method for differentiating human pluripotent stem cells to inner ear organoids that harbor functional hair cells. Using a three-dimensional culture system, we modulate TGF, BMP, FGF, and WNT signaling to generate multiple otic-vesicle-like structures from a single stem-cell aggregate. Over 2 months, the vesicles develop into inner ear organoids with sensory epithelia that are innervated by sensory neurons. Additionally, using CRISPR-Cas9, we generate an ATOH1-2A-eGFP cell line to detect hair cell induction and demonstrate that derived hair cells exhibit electrophysiological properties similar to those of native sensory hair cells. Our culture system should facilitate the study of human inner ear development and research on therapies for diseases of the inner ear. |
X Demographics
The data shown below were collected from the profiles of 166 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 42 | 25% |
| United Kingdom | 8 | 5% |
| Japan | 8 | 5% |
| France | 5 | 3% |
| Australia | 3 | 2% |
| Italy | 3 | 2% |
| Sweden | 3 | 2% |
| Netherlands | 2 | 1% |
| India | 2 | 1% |
| Other | 10 | 6% |
| Unknown | 80 | 48% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 120 | 72% |
| Scientists | 34 | 20% |
| Practitioners (doctors, other healthcare professionals) | 7 | 4% |
| Science communicators (journalists, bloggers, editors) | 5 | 3% |
Mendeley readers
The data shown below were compiled from readership statistics for 345 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Sweden | 1 | <1% |
| Germany | 1 | <1% |
| Unknown | 343 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 64 | 19% |
| Student > Ph. D. Student | 57 | 17% |
| Student > Master | 37 | 11% |
| Student > Bachelor | 37 | 11% |
| Other | 21 | 6% |
| Other | 42 | 12% |
| Unknown | 87 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 99 | 29% |
| Agricultural and Biological Sciences | 55 | 16% |
| Medicine and Dentistry | 26 | 8% |
| Neuroscience | 25 | 7% |
| Engineering | 13 | 4% |
| Other | 30 | 9% |
| Unknown | 97 | 28% |
Attention Score in Context
This research output has an Altmetric Attention Score of 272. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 April 2023.
All research outputs
#134,955
of 25,773,273 outputs
Outputs from Nature Biotechnology
#259
of 8,622 outputs
Outputs of similar age
#2,891
of 325,585 outputs
Outputs of similar age from Nature Biotechnology
#8
of 91 outputs
Altmetric has tracked 25,773,273 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 99th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 8,622 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 44.5. This one has done particularly well, scoring higher than 96% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 325,585 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 99% of its contemporaries.
We're also able to compare this research output to 91 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 91% of its contemporaries.