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Mendeley readers
| Chapter title |
ELISA for Monitoring Nerve Growth Factor
|
|---|---|
| Chapter number | 10 |
| Book title |
Molecular Profiling
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6990-6_10 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6989-0, 978-1-4939-6990-6
|
| Authors |
Justin B. Davis |
| Editors |
Virginia Espina |
| Abstract |
Nerve growth factor (NGF), a neurotrophin critical to neuronal viability, has become a popular research focus for the treatment of neurodegenerative diseases. Enzyme-linked immunosorbent assays (ELISA) allow the quantification of cellular proteins, such as NGF, secreted into conditioned culture media. However, off-the-shelf reagents and kit components may require optimization depending on the specific specimens and/or antibodies that will be utilized with the ELISA assay. Herein we describe a protocol for developing a sandwich ELISA, incorporating NGF-specific primary and secondary antibodies capable of detecting NGF at concentrations as low as 10 pg/mL. |
Mendeley readers
The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 29% |
| Student > Postgraduate | 2 | 29% |
| Professor > Associate Professor | 1 | 14% |
| Unknown | 2 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 2 | 29% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 14% |
| Immunology and Microbiology | 1 | 14% |
| Agricultural and Biological Sciences | 1 | 14% |
| Unknown | 2 | 29% |