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Assessing the potential of fish cell lines as tools for the cytotoxicity testing of estuarine sediment aqueous elutriates

Overview of attention for article published in Toxicology in Vitro, April 2005
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Title
Assessing the potential of fish cell lines as tools for the cytotoxicity testing of estuarine sediment aqueous elutriates
Published in
Toxicology in Vitro, April 2005
DOI 10.1016/j.tiv.2004.12.002
Pubmed ID
Authors

M. Davoren, S. Ní Shúilleabháin, M.G.J. Hartl, D. Sheehan, N.M. O’Brien, J. O’Halloran, F.N.A.M. Van Pelt, C. Mothersill

Abstract

In the present study, we assess the potential of fish cell lines (CHSE, EPC and RTG-2) to be used as screening tools for the ecotoxicological assessment of estuarine sediments. The processing of sediment to a form suitable for in vitro exposure is an inherent problem when using cell cultures. The approach employed in this study was to prepare aqueous elutriate extracts from whole sediments, which were subsequently used to reconstitute powdered media. This procedure allowed the exposure of cell cultures to concentrations of up to and including 100% of the original aqueous sample. Cytotoxicity was assessed using multiple endpoint measurements. Cell viability was quantified using the neutral red and alamar blue colorimetric assays, which specifically assess lysosomal and mitochondrial function, respectively. In addition, the total protein content of the cells was measured using the coomassie blue assay. Initial tests were conducted to ensure that any resultant cytotoxicity was due to sample contaminants and not osmotic stress. In addition, elutriate samples were spiked with a model toxicant to verify the ability of the cell lines to detect and respond to bioavailable contaminants. Chemical analyses were conducted on sediments from all sampling sites to assist in interpreting any observed cytotoxicity. A differential response was observed for the cytotoxicity assays following exposure treatments, which emphasises the importance of employing multiple endpoints for the determination of toxicity. Of the three cell lines utilised in this study, RTG-2 cells were the most suitable for the testing of estuarine aqueous elutriate samples on the basis of tolerance to osmolality effects. Slight toxicity was observed following exposure to the aqueous elutriates tested in this study using RTG-2 cells and the alamar blue assay. In order to fully evaluate the overall sensitivity of this cell line, further research is warranted using an extensive range of test sites incorporating more polluted sediments.

Mendeley readers

The data shown below were compiled from readership statistics for 66 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Brazil 1 2%
United Kingdom 1 2%
Canada 1 2%
Argentina 1 2%
China 1 2%
United States 1 2%
Unknown 60 91%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 16 24%
Student > Master 11 17%
Researcher 10 15%
Student > Doctoral Student 9 14%
Professor 6 9%
Other 11 17%
Unknown 3 5%
Readers by discipline Count As %
Agricultural and Biological Sciences 29 44%
Environmental Science 16 24%
Biochemistry, Genetics and Molecular Biology 4 6%
Chemistry 3 5%
Medicine and Dentistry 2 3%
Other 6 9%
Unknown 6 9%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 January 2014.
All research outputs
#19,340,469
of 21,732,065 outputs
Outputs from Toxicology in Vitro
#1,448
of 1,706 outputs
Outputs of similar age
#263,372
of 305,701 outputs
Outputs of similar age from Toxicology in Vitro
#16
of 16 outputs
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So far Altmetric has tracked 1,706 research outputs from this source. They receive a mean Attention Score of 4.5. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 16 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.