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The blue fluorescent protein from Vibrio vulnificus CKM-1 is a useful reporter for plant research

Overview of attention for article published in Botanical Studies, December 2014
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Title
The blue fluorescent protein from Vibrio vulnificus CKM-1 is a useful reporter for plant research
Published in
Botanical Studies, December 2014
DOI 10.1186/s40529-014-0079-x
Pubmed ID
Authors

Jin-Min Tu, Ming-Chung Chang, Lynn LH Huang, Ching-Dong Chang, Hao-Jen Huang, Ruey-Hua Lee, Ching-Chun Chang

Abstract

The mBFP is an improved variant of NADPH-dependent blue fluorescent protein that was originally identified from the non-bioluminescent pathogenic bacteria Vibrio vulnificus CKM-1. To explore the application of mBFP in plants, the mBFP gene expression was driven by one of the three promoters, namely, leaf-specific (RbcS), hypoxia-inducible (Adh) or auxin-inducible (DR5) promoters, in different plant tissues such as leaves, roots and flowers under diverse treatments. In addition, the expressed mBFP protein was targeted to five subcellular compartments such as cytosol, endoplasmic reticulum, apoplast, chloroplast and mitochondria, respectively, in plant cells. When the mBFP was transiently expressed in the tobacco leaves and floral tissues of moth orchid, the cytosol and apoplast exhibited brighter blue fluorescence than other compartments. The recombinant mBFP-mS1C fusion protein exhibited enhanced fluorescence intensity that was correlated with more abundant RNA transcripts (1.8 fold) as compared with a control. In the root tips of horizontally grown transgenic Arabidopsis, mBFP could be induced as a reporter under hypoxia condition. Furthermore, the mBFP was localized to the expected subcellular compartments, except that dual targeting was found when the mBFP was fused with the mitochondria-targeting signal peptide. Additionally, the brightness of mBFP blue fluorescence was correlated with NADPH concentration. The NADPH-dependent blue fluorescent protein could serve as a useful reporter in plants under aerobic or hypoxic condition. However, to avoid masking the mitochondrial targeting signal, fusing mBFP as a fusion tag in the C-terminal will be better when the mBFP is applied in mitochondria trafficking study. Furthermore, mBFP might have the potential to be further adopted as a NADPH biosensor in plant cells. Future codon optimization of mBFP for plants could significantly enhance its brightness and expand its potential applications.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 27%
Student > Bachelor 3 27%
Student > Ph. D. Student 2 18%
Lecturer 1 9%
Student > Master 1 9%
Other 1 9%
Readers by discipline Count As %
Agricultural and Biological Sciences 6 55%
Biochemistry, Genetics and Molecular Biology 3 27%
Chemistry 1 9%
Engineering 1 9%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 May 2017.
All research outputs
#10,213,802
of 12,786,466 outputs
Outputs from Botanical Studies
#51
of 105 outputs
Outputs of similar age
#190,053
of 263,206 outputs
Outputs of similar age from Botanical Studies
#5
of 5 outputs
Altmetric has tracked 12,786,466 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 105 research outputs from this source. They receive a mean Attention Score of 2.3. This one is in the 28th percentile – i.e., 28% of its peers scored the same or lower than it.
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