Title |
Silver nanoparticles: Evaluation of DNA damage, toxicity and functional impairment in human mesenchymal stem cells
|
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Published in |
Toxicology Letters, December 2010
|
DOI | 10.1016/j.toxlet.2010.12.001 |
Pubmed ID | |
Authors |
Stephan Hackenberg, Agmal Scherzed, Michael Kessler, Silke Hummel, Antje Technau, Katrin Froelich, Christian Ginzkey, Christian Koehler, Rudolf Hagen, Norbert Kleinsasser |
Abstract |
Silver nanoparticles (Ag-NPs) are the most frequent commercialized nanomaterial currently. Due to a distinct lack of information on hazardous properties of Ag-NPs in human cells, a study was conducted to evaluate Ag-NP induced DNA damage, cell death and functional impairment in human mesenchymal stem cells (hMSCs). Initially, Ag-NPs and their cellular distribution were characterized by transmission electron microscopy (TEM). Ag-NPs were exposed to hMSCs for 1, 3 and 24h. Cytotoxicity was measured by the trypan blue exclusion test and the fluorescein-diacetate test, DNA damage was evaluated by the comet assay and chromosomal aberration test. Cytokine release of IL-6, IL-8 and VEGF was observed using the ELISA technique. Additionally, hMSC migration capability was tested in a transwell system. TEM revealed a Ag-NP distribution to cytoplasm and nucleus. Cytotoxic effects were seen at concentrations of 10 μg/ml for all test exposure periods. Both, comet assay and chromosomal aberration test showed DNA damage after 1, 3, and 24h at 0.1 μg/ml. A significant increase of IL-6, IL-8 and VEGF release indicates hMSC activation. Migration ability was not impaired at subtoxic concentrations. In conclusion, we demonstrated cyto- and genotoxic potential of Ag-NPs in hMSCs at significantly higher concentrations as compared to antimicrobial effective levels. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Nigeria | 2 | 50% |
Canada | 1 | 25% |
Unknown | 1 | 25% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 4 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Portugal | 4 | 1% |
India | 3 | <1% |
United Kingdom | 2 | <1% |
Brazil | 1 | <1% |
Australia | 1 | <1% |
Germany | 1 | <1% |
Singapore | 1 | <1% |
Belgium | 1 | <1% |
Philippines | 1 | <1% |
Other | 1 | <1% |
Unknown | 325 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 80 | 23% |
Student > Master | 53 | 16% |
Researcher | 36 | 11% |
Student > Bachelor | 34 | 10% |
Student > Doctoral Student | 22 | 6% |
Other | 64 | 19% |
Unknown | 52 | 15% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 94 | 28% |
Biochemistry, Genetics and Molecular Biology | 37 | 11% |
Chemistry | 31 | 9% |
Medicine and Dentistry | 21 | 6% |
Materials Science | 18 | 5% |
Other | 64 | 19% |
Unknown | 76 | 22% |