| Chapter title |
Rescue of Infectious Salmon Anemia Virus (ISAV) from Cloned cDNA
|
|---|---|
| Chapter number | 15 |
| Book title |
Reverse Genetics of RNA Viruses
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6964-7_15 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6962-3, 978-1-4939-6964-7
|
| Authors |
Daniela Toro-Ascuy, Marcelo Cortez-San Martín, Toro-Ascuy, Daniela, Martín, Marcelo Cortez-San |
| Editors |
Daniel R. Perez |
| Abstract |
The piscine Orthomyxovirus called Infectious Salmon Anemia Virus (ISAV) is one of the most important emerging pathogens affecting the salmon industry worldwide. The first reverse genetics system for ISAV, which allows the generation of recombinant ISA virus (rISAV), is an important tool for the characterization and study of this fish virus. The plasmid-based reverse genetics system for ISAV includes the use of a novel fish promoter, the Atlantic salmon internal transcribed spacer region 1 (ITS-1). The salmon, viral and mammalian genetic elements included in pSS-URG vectors allow the expression of the eight viral RNA segments. In addition to four cytomegalovirus (CMV)-based vectors that express the four proteins of the ISAV ribonucleoprotein complex, the eight pSS-URG vectors allowed the generation of infectious rISAV in salmon cells. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 3 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 1 | 33% |
| Student > Bachelor | 1 | 33% |
| Researcher | 1 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 67% |
| Immunology and Microbiology | 1 | 33% |