Title |
Presence of infectious agents and co-infections in diarrheic dogs determined with a real-time polymerase chain reaction-based panel
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Published in |
BMC Veterinary Research, January 2014
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DOI | 10.1186/1746-6148-10-23 |
Pubmed ID | |
Authors |
Aline Baumann da Rocha Gizzi, Simone Tostes Oliveira, Christian M Leutenegger, Marko Estrada, Denise Adamczyk Kozemjakin, Rafael Stedile, Mary Marcondes, Alexander Welker Biondo |
Abstract |
Infectious diarrhea can be caused by bacteria, viruses, or protozoan organisms, or a combination of these. The identification of co-infections in dogs is important to determine the prognosis and to plan strategies for their treatment and prophylaxis. Although many pathogens have been individually detected with real-time polymerase chain reaction (PCR), a comprehensive panel of agents that cause diarrhea in privately owned dogs has not yet been established. The objective of this study was to use a real-time PCR diarrhea panel to survey the frequencies of pathogens and co-infections in owned dogs attended in a veterinary hospital with and without diarrhea, as well the frequency in different countries. Feces samples were tested for canine distemper virus, canine coronavirus, canine parvovirus type 2 (CPV-2), Clostridium perfringens alpha toxin (CPA), Cryptosporidium spp., Giardia spp., and Salmonella spp. using molecular techniques. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 50% |
Scientists | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Brazil | 2 | 2% |
Unknown | 120 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 25 | 20% |
Researcher | 14 | 11% |
Student > Bachelor | 13 | 11% |
Student > Doctoral Student | 10 | 8% |
Student > Ph. D. Student | 10 | 8% |
Other | 28 | 23% |
Unknown | 22 | 18% |
Readers by discipline | Count | As % |
---|---|---|
Veterinary Science and Veterinary Medicine | 40 | 33% |
Agricultural and Biological Sciences | 25 | 20% |
Biochemistry, Genetics and Molecular Biology | 9 | 7% |
Medicine and Dentistry | 9 | 7% |
Nursing and Health Professions | 3 | 2% |
Other | 12 | 10% |
Unknown | 24 | 20% |